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Biochemistry (Moscow)

, Volume 80, Issue 7, pp 943–956 | Cite as

X-ray reflectivity and grazing incidence diffraction studies of interaction between human adhesion/growth-regulatory galectin-1 and DPPE—GM1 lipid monolayer at an air/water interface

  • J. Majewski
  • S. André
  • E. Jones
  • E. Chi
  • H.-J. GabiusEmail author
Article

Abstract

The specific interaction of ganglioside GM1 with the homodimeric (prototype) endogenous lectin galectin-1 triggers growth regulation in tumor and activated effector T cells. This proven biorelevance directed interest to studying association of the lectin to a model surface, i.e. a 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine/ganglioside GM1 (80: 20 mol%) monolayer, at a bioeffective concentration. Surface expansion by the lectin insertion was detected at a surface pressure of 20 mN/m. On combining the methods of grazing incidence X-ray diffraction and X-ray reflectivity, a transient decrease in lipid-ordered phase of the monolayer was observed. The measured electron density distribution indicated that galectin-1 is oriented with its long axis in the surface plane, ideal for cis-crosslinking. The data reveal a conspicuous difference to the way the pentameric lectin part of the cholera toxin, another GM1-specific lectin, is bound to the monolayer. They also encourage further efforts to monitor effects of structurally different members of the galectin family such as the functionally antagonistic chimera-type galectin-3.

Keywords

agglutinin Bragg peaks ganglioside lectin X-ray diffraction/reflectivity 

Abbreviation

Ctx

cholera toxin pentamer

DPPE

1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine

Gal

galectin

GIXD

grazing incidence X-ray diffraction

LC phase

lipid-condensed phase

PSD

position-sensitive detector

XR

X-ray reflectivity

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • J. Majewski
    • 1
  • S. André
    • 2
  • E. Jones
    • 3
  • E. Chi
    • 3
  • H.-J. Gabius
    • 2
    Email author
  1. 1.Neutron Scattering Center, Los Alamos Neutron Science CenterLos Alamos National LaboratoryLos AlamosUSA
  2. 2.Institute of Physiological Chemistry, Faculty of Veterinary MedicineLudwig-Maximilians-University MunichMünchenGermany
  3. 3.Department of Chemical and Biological Engineering, Center for Biomedical EngineeringUniversity of New MexicoAlbuquerqueUSA

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