Biochemistry (Moscow)

, Volume 80, Issue 7, pp 925–933 | Cite as

Influence of fucoidans and their derivatives on antitumor and phagocytic activity of human blood leucocytes

  • N. Yu. AnisimovaEmail author
  • N. E. UstyuzhaninaEmail author
  • F. V. Donenko
  • M. I. Bilan
  • N. A. Ushakova
  • A. I. Usov
  • N. E. Nifantiev
  • M. V. Kiselevskiy


The immunotropic activity of structurally different fucoidans and their derivatives towards isolated immune blood cells, effectors of innate immune system, was studied. The most potent effect was observed for high molecular weight fucoidan CF from the alga Chordaria flagelliformis, whose backbone is built of (1→3)-linked units of α-L-fucopyranose, and branches included residues of α-D-glucuronic acid and α-L-fucofuranose. This compound at the concentration of 0.05 mg/ml potentiated phagocytosis of Saccharomyces cerevisiae and Lactobacillus acidophilus by neutrophils, increasing relative quantity of phagocytes as well as their effectiveness. Along with this, 14% increase in the concentration of membrane-bound integrin CD11c molecules was observed. The systemic effect of CF at the dose of 0.01 mg/mouse i.p. led to potentiation of cytotoxic activity of spleen mononuclear leucocytes towards melanoma cells of line B16 by 1.9-fold and towards chronic myelogenous leukemia cells of line K-562 by 1.7-fold. These results indicate that fucoidan CF can stimulate anti-infective and antitumor activity of effectors of the innate immune system via CD11c integrins.


fucoidans leucocytes natural killers phagocytosis cytotoxicity 



high molecular weight fucoidan from alga Chordaria flagelliformis


dimethyl sulfoxide


effector cells


index of cytotoxicity


mean fluorescence intensity


mononuclear leukocytes


nitroblue tetrazolium


natural killer cells


synthetic fully sulfated octasaccharide


phagocytic index


phagocytic number


dexylosylated low molecular weight fucoidan from alga Punctaria plantaginea


high molecular weight fucoidan from alga Saccharina latissima


target cells


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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • N. Yu. Anisimova
    • 1
    Email author
  • N. E. Ustyuzhanina
    • 2
    Email author
  • F. V. Donenko
    • 1
  • M. I. Bilan
    • 2
  • N. A. Ushakova
    • 3
  • A. I. Usov
    • 2
  • N. E. Nifantiev
    • 2
  • M. V. Kiselevskiy
    • 1
  1. 1.Blokhin Russian Cancer Research CenterMoscowRussia
  2. 2.Zelinsky Institute of Organic ChemistryMoscowRussia
  3. 3.Orekhovich Institute of Biomedical ChemistryMoscowRussia

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