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Biochemistry (Moscow)

, Volume 80, Issue 7, pp 915–924 | Cite as

Tissue-specific rhamnogalacturonan I forms the gel with hyperelastic properties

  • P. V. MikshinaEmail author
  • A. A. Petrova
  • D. A. Faizullin
  • Yu. F. Zuev
  • T. A. Gorshkova
Article

Abstract

Rhamnogalacturonans I are complex pectin polysaccharides extremely variable in structure and properties and widely represented in various sources. The complexity and diversity of the structure of rhamnogalacturonans I are the reasons for the limited information about the properties and supramolecular organization of these polysaccharides, including the relationship between these parameters and the functions of rhamnogalacturonans I in plant cells. In the present work, on the example of rhamnogalacturonan I from flax gelatinous fibers, the ability of this type of pectic polysaccharides to form at physiological concentrations hydrogels with hyperelastic properties was revealed for the first time. According to IR spectroscopy, water molecules are more tightly retained in the gelling rhamnogalacturonan I from flax fiber cell wall in comparison with the non-gelling rhamnogalacturonan I from primary cell wall of potato. With increase in strength of water binding by rhamnogalacturonan I, there is an increase in elastic modulus and decrease in Poisson’s ratio of gel formed by this polysaccharide. The model of hyperelastic rhamnogalacturonan I capture by laterally interacting cellulose microfibrils, constructed using the finite element method, confirmed the suitability of rhamnogalacturonan I gel with the established properties for the function in the gelatinous cell wall, allowing consideration of this tissue- and stage-specific pectic polysaccharide as an important factor in creation of gelatinous fiber contractility.

Keywords

gelatinous fibers polysaccharides rhamnogalacturonan I water sorption gel elastoplastic properties 

Abbreviation

RGf

flax fiber rhamnogalacturonan I before incorporation into the cell wall

RGfcw

rhamnogalacturonan I of flax fiber cell wall

RGp

rhamnogalacturonan I of potato primary cell wall

RH

relative humidity

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • P. V. Mikshina
    • 1
    Email author
  • A. A. Petrova
    • 1
  • D. A. Faizullin
    • 1
  • Yu. F. Zuev
    • 1
  • T. A. Gorshkova
    • 1
  1. 1.Kazan Institute of Biochemistry and Biophysics, Kazan Scientific CenterRussian Academy of SciencesKazanRussia

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