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Biochemistry (Moscow)

, Volume 80, Issue 6, pp 790–799 | Cite as

Expression of neuronal and signaling proteins in penumbra around a photothrombotic infarction core in rat cerebral cortex

  • S. V. Demyanenko
  • S. N. Panchenko
  • A. B. UzdenskyEmail author
Article

Abstract

Photodynamic impact on animal cerebral cortex using water-soluble Bengal Rose as a photosensitizer, which does not cross the blood-brain barrier and remains in blood vessels, induces platelet aggregation, vessel occlusion, and brain tissue infarction. This reproduces ischemic stroke. Irreversible cell damage within the infarction core propagates to adjacent tissue and forms a transition zone — the penumbra. Tissue necrosis in the infarction core is too fast (minutes) to be prevented, but much slower penumbral injury (hours) can be limited. We studied the changes in morphology and protein expression profile in penumbra 1 h after local photothrombotic infarction induced by laser irradiation of the cerebral cortex after Bengal Rose administration. Morphological study using standard hematoxylin/eosin staining showed a 3-mm infarct core surrounded by 1.5–2.0 mm penumbra. Morphological changes in the penumbra were lesser and decreased towards its periphery. Antibody microarrays against 224 neuronal and signaling proteins were used for proteomic study. The observed upregulation of penumbra proteins involved in maintaining neurite integrity and guidance (NAV3, MAP1, CRMP2, PMP22); intercellular interactions (N-cadherin); synaptic transmission (glutamate decarboxylase, tryptophan hydroxylase, Munc-18-1, Munc-18-3, and synphilin-1); mitochondria quality control and mitophagy (PINK1 and Parkin); ubiquitin-mediated proteolysis and tissue clearance (UCHL1, PINK1, Parkin, synphilin-1); and signaling proteins (PKBα and ERK5) could be associated with tissue recovery. Downregulation of PKC, PKCβ1/2, and TDP-43 could also reduce tissue injury. These changes in expression of some neuronal proteins were directed mainly to protection and tissue recovery in the penumbra. Some upregulated proteins might serve as markers of protection processes in a penumbra.

Key words

stroke neurodegeneration penumbra proteomics 

Abbreviations

CRMP2

collapsin response mediator protein 2

DYRK1A

dual-specificity tyrosine-phosphorylated regulated kinase 1A

ERK5

extracellular regulated kinase 2

GABA

γ-butyric acid

MAP1

microtubule-associated protein 1

NAV3

neuron navigator 3 protein

PINK1

PTEN-induced mitochondrial protein kinase

PKBβ1

protein kinase Bα

PKC

protein kinase C

PKCβ1

protein kinase C isoform β1

PMP22

peripheral myelin protein 22

PTI

photothrombotic infarction

SIRT1

NAD+-dependent deacetylase sirtuin-1

TDP-43

transactivation response DNA-binding protein

UCHL1

ubiquitin C-terminal hydrolase L1

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • S. V. Demyanenko
    • 1
  • S. N. Panchenko
    • 2
  • A. B. Uzdensky
    • 1
    Email author
  1. 1.Academy of Biology and BiotechnologySouthern Federal UniversityRostov-on-DonRussia
  2. 2.Rostov State Medical UniversityRostov-on-DonRussia

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