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Biochemistry (Moscow)

, Volume 80, Issue 6, pp 688–700 | Cite as

ESR — A retinal protein with unusual properties from Exiguobacterium sibiricum

  • L. E. PetrovskayaEmail author
  • S. P. Balashov
  • E. P. Lukashev
  • E. S. Imasheva
  • I. Yu. Gushchin
  • A. K. Dioumaev
  • A. B. Rubin
  • D. A. Dolgikh
  • V. I. Gordeliy
  • J. K. Lanyi
  • M. P. Kirpichnikov
Review

Abstract

This review covers the properties of a retinal protein (ESR) from the psychrotrophic bacterium Exiguobacterium sibiricum that functions as a light-driven proton pump. The presence of a lysine residue at the position corresponding to intramolecular proton donor for the Schiff base represents a unique structural feature of ESR. We have shown that Lys96 successfully facilitates delivery of protons from the cytoplasmic surface to the Schiff base, thus acting as a proton donor in ESR. Since proton uptake during the photocycle precedes Schiff base reprotonation, we conclude that this residue is initially in the uncharged state and acquires a proton for a short time after Schiff base deprotonation and M intermediate formation. Involvement of Lys as a proton donor distinguishes ESR from the related retinal proteins — bacteriorhodopsin (BR), proteorhodopsin (PR), and xanthorhodopsin (XR), in which the donor function is performed by residues with a carboxyl side chain. Like other eubacterial proton pumps (PR and XR), ESR contains a histidine residue interacting with the proton acceptor Asp85. In contrast to PR, this interaction leads to shift of the acceptor’s pK a to more acidic pH, thus providing its ability to function over a wide pH range. The presence of a strong H-bond between Asp85 and His57, the structure of the proton-conducting pathways from cytoplasmic surface to the Schiff base and to extracellular surface, and other properties of ESR were demonstrated by solving its three-dimensional structure, which revealed several differences from known structures of BR and XR. The structure of ESR, its photocycle, and proton transfer reactions are discussed in comparison with homologous retinal proteins.

Key words

retinal protein proteorhodopsin Exiguobacterium sibiricum Schiff base proton acceptor proton donor photocycle 

Abbreviations

BR

bacteriorhodopsin from Halobacterium salinarum

DDM

n-dodecyl-β-D-maltopyranoside

ESR

retinal protein from Exiguobacterium sibiricum

GPCR

G-protein-coupled receptors

GR

xanthorhodopsin from Gloeobacter violaceus

LPG

1-palmitoyl-2-hydroxy-sn-glycero-3-phospho-(12-rac-glycerol)

PR

proteorhodopsin

XR

xanthorhodopsin from Salinibacter ruber

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • L. E. Petrovskaya
    • 1
    Email author
  • S. P. Balashov
    • 2
  • E. P. Lukashev
    • 3
  • E. S. Imasheva
    • 2
  • I. Yu. Gushchin
    • 4
    • 5
    • 6
    • 7
    • 8
  • A. K. Dioumaev
    • 2
  • A. B. Rubin
    • 3
  • D. A. Dolgikh
    • 1
    • 3
  • V. I. Gordeliy
    • 4
    • 5
    • 6
    • 7
    • 8
  • J. K. Lanyi
    • 2
  • M. P. Kirpichnikov
    • 1
    • 3
  1. 1.Shemyakin—Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Department of Physiology and BiophysicsUniversity of CaliforniaIrvineUSA
  3. 3.Biological FacultyLomonosov Moscow State UniversityMoscowRussia
  4. 4.Institut de Biologie StructuraleUniversité Grenoble AlpesGrenobleFrance
  5. 5.Centre National de la Recherche ScientifiqueInstitut de Biologie StructuraleGrenobleFrance
  6. 6.Direction des Sciences du Vivant, Commissariat a l’Énergie AtomiqueInstitut de Biologie StructuraleGrenobleFrance
  7. 7.Laboratory for Advanced Studies of Membrane ProteinsMoscow Institute of Physics and TechnologyDolgoprudniy, Moscow RegionRussia
  8. 8.Institute of Complex Systems (ICS), ICS-6: Structural BiochemistryResearch Centre JülichJülichGermany

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