Biochemistry (Moscow)

, Volume 80, Issue 5, pp 629–635 | Cite as

Pure mitochondrial DNA does not activate human neutrophils in vitro

  • A. S. Prikhodko
  • A. K. Shabanov
  • L. A. Zinovkina
  • E. N. Popova
  • M. A. Aznauryan
  • N. O. Lanina
  • M. V. Vitushkina
  • R. A. Zinovkin
Article

Abstract

Excessive activation of the innate immune system often leads to fatal consequences and can be considered as one of the phenoptotic events. After traumatic injury, various components of mitochondria are released into the circulation and stimulate myeloid cells of the innate immunity. Presumably, mitochondrial DNA (mtDNA) might activate immune cells (Zhang, Q., et al. (2010) Nature, 464, 104–107). In the present study, we investigated the role of mtDNA as a direct activator of human neutrophils, as well as a prognostic marker in patients with severe trauma. Quantitative determination of mtDNA in the plasma of these patients revealed its significant increase (p < 0.02) in the group of survivors compared to nonsurvivors. Highly purified mtDNA was not able to induce activation of human neutrophils, thus possibly indicating the existence of additional factor(s) ensuring the recognition of mtDNA as a damage-associated molecular pattern.

Key words

trauma extracellular DNA mitochondrial DNA neutrophil activation damage-associated molecular patterns (DAMPs) 

Abbreviations

DAMP

damage-associated molecular patterns

eDNA

extracellular DNA

fMLP

formyl-Met-Leu-Phe

ISS

injury severity score

MMP9

matrix metalloproteinase 9 (gelatinase)

MTD

mitochondrial DAMP

mtDNA

mitochondrial DNA

nDNA

nuclear DNA

P-p38

phosphorylated form of p38 MAPK

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • A. S. Prikhodko
    • 1
  • A. K. Shabanov
    • 2
  • L. A. Zinovkina
    • 1
  • E. N. Popova
    • 3
  • M. A. Aznauryan
    • 1
  • N. O. Lanina
    • 1
  • M. V. Vitushkina
    • 4
  • R. A. Zinovkin
    • 3
    • 4
  1. 1.Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia
  2. 2.Sklifosovsky Research Institute of Emergency CareMoscowRussia
  3. 3.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  4. 4.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia

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