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Biochemistry (Moscow)

, Volume 80, Issue 4, pp 408–416 | Cite as

Recombinant horseradish peroxidase: Production and analytical applications

  • V. G. GrigorenkoEmail author
  • I. P. Andreeva
  • M. Yu. Rubtsova
  • A. M. Egorov
Review

Abstract

Horseradish peroxidase is a key enzyme in bio- and immunochemical analysis. New approaches in functional expression of the peroxidase gene in E. coli cells and the subsequent refolding of the resulting protein yield a recombinant enzyme that is comparable in its spectral and catalytic characteristics to the native plant peroxidase. Genetic engineering approaches allow production of recombinant peroxidase conjugates with both protein antigens and Fab antibody fragments. The present article reviews the use of recombinant horseradish peroxidase as the marker enzyme in ELISA procedures as well as in amperometric sensors based on direct electron transfer.

Key words

horseradish peroxidase recombinant conjugate amperometric biosensor 

Abbreviations

FABP

fatty acid-binding protein

HRP

horseradish peroxidase

LO

L-lysine-α-oxidase

rHRP

recombinant horseradish peroxidase

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • V. G. Grigorenko
    • 1
    Email author
  • I. P. Andreeva
    • 1
  • M. Yu. Rubtsova
    • 1
  • A. M. Egorov
    • 1
    • 2
  1. 1.Chemical FacultyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Microbiology DepartmentRussian Medical Academy of Post-Graduate EducationMoscowRussia

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