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Biochemistry (Moscow)

, Volume 80, Issue 3, pp 374–378 | Cite as

Normal level of sepsis-associated phenylcarboxylic acids in human serum

  • N. V. BeloborodovaEmail author
  • V. V. Moroz
  • A. A. Osipov
  • A. Yu. Bedova
  • A. Yu. Olenin
  • M. L. Getsina
  • O. V. Karpova
  • E. G. Olenina
Article

Abstract

Previous studies showed that large amounts of phenylcarboxylic acids (PhCAs) are accumulated in a septic patient’s blood due to increased endogenous and microbial phenylalanine and tyrosine biotransformation. Frequently, biochemical aromatic amino acid transformation into PhCAs is considered functionally insignificant for people without monogenetic hereditary diseases. The blood of healthy people contains the same PhCAs that are typical for septic patients as shown in this paper. The overall serum PhCAs level was 6 μM on average as measured by gas chromatography with flame ionization detection. This level is a stable biochemical parameter indicating the normal metabolism of aromatic amino acids. The concentrations of PhCAs in the metabolic profile of healthy people are distributed as follows: phenylacetic ≈ p-hydroxyphenyllactic > p-hydroxyphenylacetic > phenyllactic ≈ phenylpropionic > benzoic. We conclude that maintaining of stable PhCAs level in the serum is provided as the result of integration of human endogenous metabolic pathways and microbiota.

Key words

tyrosine metabolism sepsis phenylcarboxylic acids phenyllactic acid p-hydroxyphenyllactic acid phenylpropionic acid benzoic acid microbiota 

Abbreviations

BA

benzoic acid

3,4-DHBA

3,4-dihydroxybenzoic acid

GC-FID

gas chromatography with flame ionization detection

GC-MS

gas chromatography coupled with mass spectrometry

IR

interquartile range

PAA

phenylacetic acid

PhCAs

phenylcarboxylic acids

p-HPAA

p-hydroxyphenylacetic acid

p-HPPA

p-hydroxyphenylpropionic acid

p-HPLA

p-hydroxyphenyllactic acid

PLA

phenyllactic acid

PPA

phenylpropionic acid

PR

percentile range

TMS

trimethylsilyl

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • N. V. Beloborodova
    • 1
    Email author
  • V. V. Moroz
    • 1
  • A. A. Osipov
    • 1
    • 2
  • A. Yu. Bedova
    • 1
  • A. Yu. Olenin
    • 1
  • M. L. Getsina
    • 1
  • O. V. Karpova
    • 2
  • E. G. Olenina
    • 3
  1. 1.Negovsky Research Institute of General ReanimatologyMoscowRussia
  2. 2.Dmitry Rogachev Federal Scientific Clinical Center of Pediatric Hematology, Oncology, and ImmunologyMinistry of Health of the Russian FederationMoscowRussia
  3. 3.All-Russian Research Institute of Metrological ServiceMoscowRussia

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