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Biochemistry (Moscow)

, Volume 80, Issue 3, pp 276–283 | Cite as

Downregulation of miR-221, -30d, and -15a contributes to pathogenesis of prostate cancer by targeting Bmi-1

  • Hanqing Xuan
  • Wei XueEmail author
  • Jiahua Pan
  • Jianjun Sha
  • Baijun Dong
  • Yiran Huang
Article

Abstract

Prostate cancer is the second leading cause of cancer-related deaths of men. Bmi-1, a member of PcG family of proteins, has been implicated in the pathogenesis of prostate cancer, and disturbed profile of microRNAs (miRNAs) has been found in prostate cancer tissues. How Bmi-1 is regulated by miRNAs is unclear. In this study, we screened 18 miRNAs that potentially repress the expression of Bmi-1 using a dual luciferase system and found that 12 miRNAs could bind with the 3′-untranslated region of Bmi-1 mRNA. Using qRT-PCR, we found that expression of miR-221, -15a, and -30d was significantly reduced in prostate cancer tissues. Subsequent functional study indicated that miR-221 and miR-30d can repress prostate cancer cell proliferation, and this effect can be partially rescued by Bmi-1 overexpression. Our study constructs the relation between downregulated miR-221 and miR-30d and prostate cancer pathogenesis. These results indicate that miR-221 and miR-30d are candidate tumor suppressor miRNAs in prostate cancer and therefore serve as potential clinical classification markers and therapeutic targets for human prostate cancer.

Key words

miRNA prostate cancer Bmi-1 cell proliferation 

Abbreviations

AGO2

Argonaute RISC catalytic component 2

Bmi-1

B-cell specific Moloney leukemia virus insertion region homolog 1 (oncoprotein from PcG (polycomb group))

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

miRNA

microRNA

PCa

prostate cancer

siRNA

small interfering RNA

3′-UTR

3′-untranslated region

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • Hanqing Xuan
    • 1
  • Wei Xue
    • 1
    Email author
  • Jiahua Pan
    • 1
  • Jianjun Sha
    • 1
  • Baijun Dong
    • 1
  • Yiran Huang
    • 1
  1. 1.Department of Urology, Ren Ji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina

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