Biochemistry (Moscow)

, Volume 80, Issue 2, pp 208–218 | Cite as

Reorganization of low-molecular-weight fraction of plasma proteins in the annual cycle of cyprinidae

  • A. M. AndreevaEmail author
  • N. E. Lamas
  • M. V. Serebryakova
  • I. P. Ryabtseva
  • V. V. Bolshakov


Reorganization of the low-molecular-weight fraction of cyprinid plasma was analyzed using various electrophoretic techniques (disc electrophoresis, electrophoresis in polyacrylamide concentration gradient, in polyacrylamide with urea, and in SDS-polyacrylamide). The study revealed coordinated changes in the low-molecular-weight protein fractions with seasonal dynamics and related reproductive rhythms of fishes. We used cultured species of the Cyprinidae family with sequenced genomes for the detection of these interrelations in fresh-water and anadromous cyprinid species. The common features of organization of fish low-molecular-weight plasma protein fractions made it possible to make reliable identification of their proteins. MALDI mass-spectrometry analysis revealed the presence of the same proteins (hemopexin, apolipoproteins, and serpins) in the low-molecular-weight plasma fraction in wild species and cultured species with sequenced genomes (carp, zebrafish). It is found that the proteins of the first two classes are organized as complexes made of protein oligomers. Stoichiometry of these complexes changes in concordance with the seasonal and reproductive rhythms.

Key words

fish plasma proteins mass spectra MALDI 


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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • A. M. Andreeva
    • 1
    Email author
  • N. E. Lamas
    • 2
    • 3
  • M. V. Serebryakova
    • 4
  • I. P. Ryabtseva
    • 1
  • V. V. Bolshakov
    • 1
  1. 1.Papanin Institute for Biology of Inland WatersRussian Academy of SciencesBorokRussia
  2. 2.Zhirmunsky Institute of Marine BiologyFar-Eastern Branch of the Russian Academy of SciencesVladivostokRussia
  3. 3.Far-Earstern Federal UniversityVladivostokRussia
  4. 4.Lomonosov Moscow State UniversityMoscowRussia

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