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Biochemistry (Moscow)

, Volume 80, Issue 1, pp 61–66 | Cite as

Effect of trehalose on oxygen evolution and electron transfer in photosystem 2 complexes

  • M. D. MamedovEmail author
  • I. O. Petrova
  • D. V. Yanykin
  • A. A. Zaspa
  • A. Yu. Semenov
Article

Abstract

The pigment-protein complex of photosystem 2 (PS 2) catalyzes the light-driven oxidation of water molecule and the reduction of plastoquinone. In this work, we studied the effect of the disaccharide trehalose, which is unique in its physicochemical properties, on isolated PS 2 complex. It was found that trehalose significantly stimulated the steady-state rate of oxygen evolution. The study of single flash-induced fluorescence decay kinetics demonstrated that trehalose did not affect the rate of Q A oxidation, although it led to an increase in the relative fractions of PS 2 reaction centers capable of Q A oxidation. Trehalose also prevented PS 2 complexes from being inactivated on prolonged storage. We propose that in the presence of trehalose, which affects the extent of hydration, the protein can preferentially exist in a more optimal conformation for effective functioning.

Key words

photosystem 2 water-oxidizing complex oxygen evolution trehalose chlorophyll fluorescence plastoquinone oxidation 

Abbreviations

DCBQ

2,6-dichloro-p-benzoquinone

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

P680

primary electron donor

PS 2

photosystem 2

QA and QB

primary and secondary quinone acceptors

RC

reaction center

WOC

water-oxidizing complex

YZ

redox-active tyrosine 161 of D1 protein

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • M. D. Mamedov
    • 1
    • 2
    Email author
  • I. O. Petrova
    • 1
  • D. V. Yanykin
    • 2
  • A. A. Zaspa
    • 1
  • A. Yu. Semenov
    • 1
    • 3
  1. 1.Belozersky Institute of Physical-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia
  3. 3.Semenov Institute of Chemical PhysicsRussian Academy of SciencesMoscowRussia

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