Biochemistry (Moscow)

, Volume 79, Issue 12, pp 1339–1348 | Cite as

Bacterial lipopolysaccharide activates CD57-negative human NK cells

  • L. M. Kanevskiy
  • S. A. Erokhina
  • M. A. Streltsova
  • W. G. Telford
  • A. M. Sapozhnikov
  • E. I. Kovalenko
Article

Abstract

NK cells play an important regulatory role in sepsis by induction and augmentation of proinflammatory reactions in early stages of the septic process and by suppression of immune response in later stages of inflammation. The present work was aimed at the effect of bacterial lipopolysaccharide (LPS), the main pathogenic factor of sepsis development, on human NK cells ex vivo. We show that LPS activates immature CD57-negative NK cells, which typically constitute less than half of the normal NK cell population in human peripheral blood. Under conditions of NK cell stimulation with IL-2, addition of LPS provokes an increase in IFN-γ production. However, LPS both increased and inhibited NK cell cytotoxic activity. It is important to note that the activation of NK cells on LPS addition was observed in the absence of TLR4 on the NK cell surface. These results confirm our previous data arguing for a direct interaction of LPS with NK cells and evidence an atypical mechanism of LPS-induced NK cell activation without the involvement of surface TLR4.

Key words

NK cells lipopolysaccharide IFN-γ production CD57 cytotoxicity TLR4 

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • L. M. Kanevskiy
    • 1
  • S. A. Erokhina
    • 1
    • 2
  • M. A. Streltsova
    • 1
  • W. G. Telford
    • 3
  • A. M. Sapozhnikov
    • 1
    • 2
  • E. I. Kovalenko
    • 1
  1. 1.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Experimental Transplantation and Immunology Branch, National Cancer InstituteNational Institutes of HealthBethesdaUSA

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