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Biochemistry (Moscow)

, Volume 79, Issue 11, pp 1216–1225 | Cite as

Effect of naphthalene on photosystem 2 photochemical activity of pea plants

  • A. V. Lankin
  • V. D. KreslavskiEmail author
  • A. Yu. Khudyakova
  • S. K. ZharmukhamedovEmail author
  • S. I. AllakhverdievEmail author
Article

Abstract

The effect of a typical polyaromatic hydrocarbon, naphthalene (Naph), on photosystem 2 (PS-2) photochemical activity in thylakoid membrane preparations and 20-day-old pea leaves was studied. Samples were incubated in water in the presence of Naph (0.078, 0.21, and 0.78 mM) for 0.5–24 h under white light illumination (15 μmol photons·m−2·s−1). The PS-2 activity was determined by studying fast and delayed chlorophyll (Chl) a fluorescence. Incubation of samples in water solutions at Naph concentrations of 0.21 and 0.78 mM led to a decrease in the maximum PS-2 quantum efficiency (Fv/Fm), noticeable changes in the polyphasic induction kinetics of fluorescence (OJIP), and a decrease in the amplitudes of the fast and slow components of delayed fluorescence of Chl a. The rate of release of electrolytes from leaves that were preliminarily incubated with Naph (0.21 mM) was also increased. Significant decrease in the fluorescence parameters in thylakoid membrane preparations was observed at Naph concentration of 0.03 mM and 12-min exposure of the samples. Chlorophyll (a and b) and carotenoid content (mg per gram wet mass) was insignificantly changed. The quantum yields of electron transfer from QA to QBET2o) and also to the PS-1 acceptors (φRE1o) were reduced. These results are explained by the increase in the number of QB-non-reducing centers of PS-2, which increased with increasing Naph concentration and exposure time of leaves in Naph solution. The suppression of PS-2 activity was partly abolished in the presence of the electron donor sodium ascorbate. Based on these results, it is suggested that Naph distorts cell membrane intactness and acts mainly on the PS-2 acceptor and to a lesser degree on the PS-2 donor side.

Key words

naphthalene photosystem 2 variable and delayed fluorescence of chlorophyll detached pea leaves thylakoid membranes photosynthetic pigments 

Abbreviations

Asc

sodium ascorbate

Chl

chlorophyll

DFl

delayed fluorescence

Fo

minimum fluorescence

Fm

maximum fluorescence

Fv

variable fluorescence (Fv = Fo − Fm)

FFl

fast fluorescence

Naph

naphthalene

PA

photosynthetic apparatus

PAHs

polycyclic aromatic hydrocarbons

PS-2

photosystem 2

QA

primary quinone acceptor of PS-2

QB

secondary quinone acceptor of PS-2

RC

reaction center

TM

thylakoid membranes

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia
  3. 3.Institute of Biological EngineeringRussian Academy of SciencesPushchino, Moscow RegionRussia

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