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Biochemistry (Moscow)

, Volume 79, Issue 7, pp 663–671 | Cite as

Secretion of adipocytes and macrophages under conditions of inflammation and/or insulin resistance and effect of adipocytes on preadipocytes under these conditions

  • Yu-Tao Wei
  • Dong-Sheng Xia
  • Wen-Kai Yang
  • Xian-Guo Wang
  • Xin-Zhong ChenEmail author
  • Nian-Guo Dong
Article

Abstract

The purpose of the present study was to examine changes in preadipocytes following the coculture of preadipocytes and adipocytes and the effects on the secretion of adipocytes and macrophages following induction of inflammation and insulin resistance. Mature adipocytes and RAW264.7 macrophages were treated with lipopolysaccharide and insulin to establish models of inflammation and insulin resistance, respectively. The mRNA expression levels of IL-6, MCP-1, and TNF-α in all adipocyte treatment groups were significantly greater compared with the control, and that of adiponectin was less (P < 0.05). In the RAW264.7 macrophages, the mRNA expression levels of IL-6 and TNF-α were greater than those in the control group (P < 0.05). Moreover, the results of this study confirmed that adipocytes and macrophages increased the secretion of inflammatory factors under conditions of induced inflammation and insulin resistance. In addition, 3T3-L1 adipocytes inhibited the proliferation and differentiation of preadipocytes when cocultured with adipocytes under conditions of inflammation and/or insulin resistance, and the phenotype of preadipocytes did not change.

Key words

insulin resistance adipocytes preadipocytes adipokines 

Abbreviations

C/EBP

CCAAT-enhancer-binding protein

DAPI

4′,6-diamidino-2-phenylindole

IBMX

3-isobutyl-1-methylxanthine

IL-6

interleukin-6

IR

insulin resistance

MCP-1

monocyte chemotactic protein-1

MTT

methyl thiazolyl tetrazolium

PBS

phosphate-buffered saline

PID

postinduction day

PPARγ

peroxisome proliferator-activated receptor gamma

Pref-1

preadipocyte differentiation factor 1

RT-qPCR

real-time quantitative polymerase chain reaction

TNF-α

tumor necrosis factor-alpha

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • Yu-Tao Wei
    • 1
    • 2
    • 3
  • Dong-Sheng Xia
    • 3
    • 4
  • Wen-Kai Yang
    • 3
    • 5
  • Xian-Guo Wang
    • 3
  • Xin-Zhong Chen
    • 3
    Email author
  • Nian-Guo Dong
    • 3
  1. 1.Department of Thoracic and Cardiovascular SurgerySecond Hospital Affiliated with the Medical College of Shihezi University (Department of Thoracic and Cardiovascular Surgery, Hospital of Xingjian Production and Construction Corps)WulumuqiXinjiang, China
  2. 2.Department of Thoracic and Cardiovascular SurgeryFirst Hospital Affiliated with the Medical College of Shihezi UniversityShiheziXinjiang, China
  3. 3.Department of Cardiovascular Surgery, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei, China
  4. 4.Department of Cardiovascular SurgeryHenan Provincial People’s HospitalZhengzhouHenan, China
  5. 5.Department of Cardiac SurgeryJiangxi Provincial People’s HospitalNanchangJiangxi, China

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