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Biochemistry (Moscow)

, Volume 79, Issue 7, pp 637–642 | Cite as

Selective inhibitor of histone deacetylase 6 (tubastatin A) suppresses proliferation of hepatitis C virus replicon in culture of human hepatocytes

  • M. V. KozlovEmail author
  • A. A. Kleymenova
  • K. A. Konduktorov
  • A. Z. Malikova
  • S. N. Kochetkov
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Abstract

Acetylation of α-tubulin was studied in cultures of human hepatocytes under the influence of selective inhibitors of histone deacetylases HDAC6 and SIRT-2 — tubastatin A and 2-(3-phenethoxyphenylamino)benzamide, respectively. It was found that in hepatocyte cell line HepG2 acetylated α-tubulin is accumulated preferentially on inhibition of HDAC6 but not of SIRT-2. Under the same conditions, no acetylation of α-tubulin was observed in hepatocyte cell line Huh7. However, the inhibition of HDAC6 with tubastatin A led to hyperacetylation of α-tubulin and simultaneously to decrease in viral RNA concentration in hepatocyte cell line Huh7-luc/neo, which supports propagation of the full genome replicon of hepatitis C virus. The correlation between these two processes points to HDAC6 as a promising cellular target for therapy of hepatitis C.

Key words

human hepatocytes acetylation of α-tubulin HDAC6 and SIRT-2 hepatitis C virus replicon 

Abbreviations

HCV

hepatitis C virus

HDAC6

Zn2+-dependent histone deacetylase 6

MT

microtubules

SIRT-2

NAD+-dependent histone deacetylase

αTAT1

α-tubulin acetyltransferase of mammals

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • M. V. Kozlov
    • 1
    Email author
  • A. A. Kleymenova
    • 1
  • K. A. Konduktorov
    • 1
  • A. Z. Malikova
    • 2
  • S. N. Kochetkov
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Kazan Federal UniversityKazan, Republic of TatarstanRussia

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