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Biochemistry (Moscow)

, Volume 79, Issue 6, pp 538–544 | Cite as

HMGA1 is a new target of miR-195 involving isoprenaline-induced cardiomyocyte hypertrophy

  • Xiang-Yu You
  • Jiong-Hua Huang
  • Bin Liu
  • Shao-Jun Liu
  • Yun Zhong
  • Shi-Ming LiuEmail author
Article

Abstract

Emerging data have shown that microRNAs (miRNAs) have important functions in the processes of cardiac hypertrophy and heart failure that occur during the postnatal period. Cardiac overexpression of miR-195 results in pathological cardiac growth and heart failure in transgenic mice. In the present study, we analyzed the roles of miR-195 in cardiomyocyte hypertrophy and found that miR-195 was greatly upregulated during isoprenaline-induced cardiomyocyte hypertrophy. By using mRNA microarray and molecular approach, we identified a novel putative target of miR-195 called high-mobility group A1 (HMGA1). Total mRNA microarray showed that HMGA1 was downregulated in primary cardiomyocytes that overexpressed miR-195. Using luciferase activity assay, we demonstrated that miR-195 interacts with the 3′-untranslated region of HMGA1 mRNA. Moreover, we showed that miR-195 in primary cardiomyocytes downregulates the expression of HMGA1 at the protein level. Taken together, our data demonstrated that miR-195 can negatively regulate a new target, HMGA1, which is involved in cardiomyocyte hypertrophy.

Key words

microRNA-195 cardiomyocyte hypertrophy microRNA target HMGA1 

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • Xiang-Yu You
    • 1
  • Jiong-Hua Huang
    • 1
  • Bin Liu
    • 1
  • Shao-Jun Liu
    • 1
  • Yun Zhong
    • 1
  • Shi-Ming Liu
    • 1
    Email author
  1. 1.Cardiovascular DepartmentSecond Hospital Affiliated to Guangzhou Medical University, Guangzhou Institute of Cardiovascular DiseaseGuangzhouPR China

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