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Biochemistry (Moscow)

, Volume 79, Issue 5, pp 417–424 | Cite as

Calix[4]arene C-90 selectively inhibits Ca2+,Mg2+-ATPase of myometrium cell plasma membrane

  • T. A. VeklichEmail author
  • A. A. Shkrabak
  • N. N. Slinchenko
  • I. I. Mazur
  • R. V. Rodik
  • V. I. Boyko
  • V. I. Kalchenko
  • S. A. Kosterin
Article

Abstract

The supramolecular compound calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)-methylamino-25,26,27,28-tetrapropoxycalix[4]arene) is shown to efficiently inhibit the ATP hydrolase activity of Ca2+,Mg2+-ATPase in the myometrium cell plasma membrane fraction and also in a preparation of the purified enzyme solubilized from this subcellular fraction. The inhibition coefficient I 0.5 values were 20.2 ± 0.5 and 58.5 ± 6.4 μM for the membrane fraction and the solubilized enzyme, respectively. The inhibitory effect of calix[4]arene C-90 was selective comparatively to other ATPases localized in the plasma membrane: calix[4]arene C-90 did not influence the activities of Na+,K+-ATPase and “basal” Mg2+-ATPase. The inhibitory effect of calix[4]arene C-90 on the Ca2+,Mg2+-ATPase activity was associated with the cooperative action of four trifluoromethylphenyl sulfonylimine (sulfonylamidine) groups oriented similarly on the upper rim of the calix[4]arene macrocycle (the calix[4]arene “bowl”). The experimental findings seem to be of importance for studies, using calix[4]arene C-90, of membrane mechanisms of regulation of calcium homeostasis in smooth muscle cells and also for investigation of the participation of the plasma membrane Ca2+-pump in control of electro- and pharmacomechanical coupling in myocytes.

Key words

Ca2+,Mg2+-ATPase plasma membrane calcium pump smooth muscle cells myometrium calix[4]arenes sulfonylamidines 

Abbreviations

PM

plasma membrane

SMC

smooth muscular cells

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • T. A. Veklich
    • 1
    Email author
  • A. A. Shkrabak
    • 1
  • N. N. Slinchenko
    • 1
  • I. I. Mazur
    • 1
  • R. V. Rodik
    • 2
  • V. I. Boyko
    • 2
  • V. I. Kalchenko
    • 2
  • S. A. Kosterin
    • 1
  1. 1.Palladin Institute of BiochemistryNational Academy of Sciences of UkraineKievUkraine
  2. 2.Institute of Organic ChemistryNational Academy of Sciences of UkraineKievUkraine

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