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Biochemistry (Moscow)

, Volume 79, Issue 2, pp 77–87 | Cite as

Expression of exogenous DNA methyltransferases: Application in molecular and cell biology

  • O. V. Dyachenko
  • S. V. Tarlachkov
  • D. V. Marinitch
  • T. V. Shevchuk
  • Y. I. BuryanovEmail author
Review

Abstract

DNA methyltransferases might be used as powerful tools for studies in molecular and cell biology due to their ability to recognize and modify nitrogen bases in specific sequences of the genome. Methylation of the eukaryotic genome using exogenous DNA methyltransferases appears to be a promising approach for studies on chromatin structure. Currently, the development of new methods for targeted methylation of specific genetic loci using DNA methyltransferases fused with DNA-binding proteins is especially interesting. In the present review, expression of exogenous DNA methyltransferase for purposes of in vivo analysis of the functional chromatin structure along with investigation of the functional role of DNA methylation in cell processes are discussed, as well as future prospects for application of DNA methyltransferases in epigenetic therapy and in plant selection.

Key words

DNA methyltransferases targeted DNA methylation chromatin structure functions of DNA methylation 

Abbreviations

DNA-MTase

DNA methyltransferase

m5C

C5-methylcytosine

m4C

N4-methylcytosine

m6A

N6-methyladenine

PCR

polymerase chain reaction

H is A

T or C

W is

A or T

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • O. V. Dyachenko
    • 1
  • S. V. Tarlachkov
    • 1
    • 2
  • D. V. Marinitch
    • 3
  • T. V. Shevchuk
    • 1
  • Y. I. Buryanov
    • 1
    Email author
  1. 1.Branch of Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesPushchino, Moscow RegionRussia
  2. 2.Pushchino State Institute of Natural SciencesPushchino, Moscow RegionRussia
  3. 3.Republican Research and Production Center of Transfusiology and Medical BiotechnologiesMinskRepublic of Belarus

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