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Biochemistry (Moscow)

, Volume 79, Issue 1, pp 25–30 | Cite as

A novel approach for fluorescent visualization of glycyrrhetic acid on a cell with a quantum dot

  • Jie HouEmail author
  • Qian Shi
  • Meirong Cao
  • Pengwei Pan
  • Guangbo Ge
  • Xuran Fan
  • Gang Bai
  • Yi Xin
Article

Abstract

Glycyrrhetic acid (GA), a pentacyclic triterpenoid derivative obtained from hydrolysis of glycyrrhizic acid, was found to have synergistic anti-asthmatic effects with the β2-adrenergic receptor (β2AR) agonist via the β2AR-mediated pathway. This study visualized the location of GA on a human cell expressing β2AR via chemical biological approaches. A CdTe/ZnS quantum dot modified with an alkynyl group (QD-AL) was first synthesized, and an azide-terminal GA (ATGA) was also prepared. The QD-AL was used for fluorescence visualization of the distribution of GA on human embryonic kidney 293 cells expressing fusion β2AR (HEK293-β2AR) through the “click reaction” between QD-AL and ATGA. The average size of the QD-AL nanoparticle was about 10 nm, and its fluorescent emission wavelength was 620 nm. The location of GA on the HEK293-β2AR cell membrane can be visualized by the click reaction (between QD-AL and ATGA). The ability of QD-AL targeting to ATGA on the cell membrane of a HEK293-β2AR cell was further investigated using both confocal laser-scanning microscopy and a cellular uptake-inhibition assay. The results reveal that QD-AL can recognize ATGA on the cell membrane through the click reaction, which provides a new approach for visualizing the location of GA on the cell in an indirect way, and it can be applied to explore the synergistic anti-asthmatic mechanism of GA with β2AR agonist through the β2AR mediated pathway.

Key words

fluorescent visualization glycyrrhetic acid quantum dot click reaction 

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Copyright information

© Pleiades Publishing, Ltd. 2014

Authors and Affiliations

  • Jie Hou
    • 1
    • 2
    Email author
  • Qian Shi
    • 2
  • Meirong Cao
    • 2
  • Pengwei Pan
    • 2
  • Guangbo Ge
    • 3
  • Xuran Fan
    • 3
  • Gang Bai
    • 2
  • Yi Xin
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyDalian Medical UniversityDalianChina
  2. 2.College of PharmacyNankai UniversityTianjinChina
  3. 3.Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

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