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Biochemistry (Moscow)

, Volume 78, Issue 11, pp 1287–1292 | Cite as

Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein

  • M. Milivojevic
  • I. PetrovicEmail author
  • N. Kovacevic-Grujicic
  • J. Popovic
  • M. Mojsin
  • M. Stevanovic
Article

Abstract

SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.

Key words

SOX18 dominant-negative transcription factor truncated protein 

Abbreviations

HMG

high mobility group box

SOX18wt

wild-type SOX18

SOX18DN

truncated dominant-negative mutant protein SOX18

Sry gene

sex determining region of Y chromosome

TAD

trans-activation domain (of SOX18)

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • M. Milivojevic
    • 1
  • I. Petrovic
    • 1
    Email author
  • N. Kovacevic-Grujicic
    • 1
  • J. Popovic
    • 1
  • M. Mojsin
    • 1
  • M. Stevanovic
    • 1
  1. 1.Institute of Molecular Genetics and Genetic EngineeringUniversity of BelgradeBelgradeSerbia

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