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Biochemistry (Moscow)

, Volume 78, Issue 11, pp 1280–1286 | Cite as

Organotypic culture of neural retina as a research model of neurodegeneration of ganglion cells

  • O. S. GancharovaEmail author
  • V. N. Manskikh
  • A. A. ZamyatninJr.
  • P. P. Philippov
Article

Abstract

Organotypic models deserve special attention among the large variety of methods of vertebrate retina cultivation. The purpose of this study was to make a detailed qualitative and quantitative characterization of a model employing roller organotypic cultivation of the neural retina of rat eye posterior segment, with special attention to morphological and functional characteristics of retinal ganglion cells. The study included morphological analysis of retina histological preparations as well as estimation of RNA synthesis and evaluation of neuron survival by the Brachet and TUNEL methods, respectively. Retina has been shown to display normal morphofunctional characteristics for the first 12 h of cultivation. After 24 h, a substantial number of ganglion cells underwent pyknosis and stopped RNA synthesis. Almost all the cells of the retinal ganglion layer became apoptotic by 3–4 days in vitro. In the course of cultivation, neural retina is detached from the underlying layers of the posterior eye segment and undergoes significant cytoarchitectonic changes. The causes of ganglion cell death during organotypic cultivation of eye posterior segment are discussed. This method can serve as a suitable model for the screening of new retinoprotectors and for research on ganglion cell death resulting from retina degenerative diseases, e.g. glaucoma.

Key words

retina ganglion cells apoptosis organotypic roller culture 

Abbreviations

GCL

ganglion cell retina layer

INL

inner nuclear retina layer

IPL

inner plexiform retina layer

ONL

outer nuclear retina layer

OPL

outer plexiform retina layer

PRL

photoreceptor retina layer

RPE

retinal pigment epithelium

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • O. S. Gancharova
    • 1
    • 2
    • 3
    Email author
  • V. N. Manskikh
    • 1
    • 3
  • A. A. ZamyatninJr.
    • 2
    • 4
  • P. P. Philippov
    • 1
    • 2
  1. 1.Institute of MitoengineeringLomonosov Moscow State UniversityMoscowRussia
  2. 2.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Belozersky Institute of Physico-Chemical Biology, Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia
  4. 4.Institute of Molecular MedicineSechenov First Moscow State Medical UniversityMoscowRussia

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