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Biochemistry (Moscow)

, Volume 78, Issue 9, pp 1006–1012 | Cite as

Effect of mitochondria-targeted antioxidant SkQ1 on programmed cell death induced by viral proteins in tobacco plants

  • A. D. Solovieva
  • O. Yu. Frolova
  • A. G. Solovyev
  • S. Yu. Morozov
  • A. A. ZamyatninJr.Email author
Phenoptosis

Abstract

Programmed cell death (PCD) is the main defense mechanism in plants to fight various pathogens including viruses. The best-studied example of virus-induced PCD in plants is Tobacco mosaic virus (TMV)-elicited hypersensitive response in tobacco plants containing the N resistance gene. It was previously reported that the animal mitochondrial protein Bcl-xL, which lacks a homolog in plants, effectively suppresses plant PCD induced by TMV p50 — the elicitor of hyper-sensitive response in Nicotiana tabacum carrying the N gene. Our studies show that the mitochondria-targeted antioxidant SkQ1 effectively suppresses p50-induced PCD in tobacco plants. On the other hand, SkQ1 did not affect Poa semilatent virus TGB3-induced endoplasmic reticulum stress, which is followed by PCD, in Nicotiana benthamiana epidermal cells. These data suggest that mitochondria-targeted antioxidant SkQ1 can be used to study molecular mechanisms of PCD suppression in plants.

Key words

mitochondria-targeted compounds reactive oxygen species hypersensitive response ER stress unfolded protein response 

Abbreviations

C12TPP

dodecyl triphenylphosphonium

dpi

day post infiltration

ER

endoplasmic reticulum

PCD

programmed cell death

PSLV

Poa semilatent virus

PVX

potato virus X

ROS

reactive oxygen species

TMV

tobacco mosaic virus

UPR

unfolded protein response

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • A. D. Solovieva
    • 1
  • O. Yu. Frolova
    • 2
  • A. G. Solovyev
    • 3
    • 4
  • S. Yu. Morozov
    • 3
  • A. A. ZamyatninJr.
    • 3
    • 5
    Email author
  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of MitoengineeringLomonosov Moscow State UniversityMoscowRussia
  3. 3.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  4. 4.Institute of Agricultural BiotechnologyRussian Academy of Agricultural SciencesMoscowRussia
  5. 5.Institute of Molecular MedicineSechenov First Moscow State Medical UniversityMoscowRussia

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