Biochemistry (Moscow)

, Volume 78, Issue 7, pp 818–822 | Cite as

Development of approaches to creation of experimental test system for evaluation of antigenic activity of synthetic oligosaccharide ligands related to fragments of the Streptococcus pneumoniae type 14 capsular polysaccharide

  • E. A. Kurbatova
  • D. S. Vorobiov
  • I. B. Semenova
  • E. V. Sukhova
  • D. V. Yashunsky
  • Y. E. Tsvetkov
  • N. E. Nifantiev
Article

Abstract

A conjugate of a synthetic hexasaccharide fragment of the Streptococcus pneumoniae type 14 capsular polysaccharide with bovine serum albumin (BSA) has been prepared. The antigenic activity and specificity of this conjugate are comparable with those of natural antigens of S. pneumoniae type 14. The data suggest that the resulting synthetic conjugate can be used as a coating antigen in an experimental test system (based on enzyme immunoassay) for evaluating the antigenic activity and specificity of synthetic oligosaccharide ligands and for testing specimens of natural capsular polysaccharides and immune sera.

Key words

Streptococcus pneumoniae type 14 capsular polysaccharide synthetic oligosaccharide fragment oligosaccharide-BSA conjugate antigenic activity 

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • E. A. Kurbatova
    • 1
  • D. S. Vorobiov
    • 1
  • I. B. Semenova
    • 1
  • E. V. Sukhova
    • 2
  • D. V. Yashunsky
    • 2
    • 3
  • Y. E. Tsvetkov
    • 2
  • N. E. Nifantiev
    • 2
  1. 1.Mechnikov Research Institute of Vaccines and SeraRussian Academy of Medical SciencesMoscowRussia
  2. 2.Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Orekhovich Research Institute of Biomedical ChemistryRussian Academy of Medical SciencesMoscowRussia

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