Biochemistry (Moscow)

, Volume 78, Issue 7, pp 818–822 | Cite as

Development of approaches to creation of experimental test system for evaluation of antigenic activity of synthetic oligosaccharide ligands related to fragments of the Streptococcus pneumoniae type 14 capsular polysaccharide

  • E. A. Kurbatova
  • D. S. Vorobiov
  • I. B. Semenova
  • E. V. Sukhova
  • D. V. Yashunsky
  • Y. E. Tsvetkov
  • N. E. Nifantiev


A conjugate of a synthetic hexasaccharide fragment of the Streptococcus pneumoniae type 14 capsular polysaccharide with bovine serum albumin (BSA) has been prepared. The antigenic activity and specificity of this conjugate are comparable with those of natural antigens of S. pneumoniae type 14. The data suggest that the resulting synthetic conjugate can be used as a coating antigen in an experimental test system (based on enzyme immunoassay) for evaluating the antigenic activity and specificity of synthetic oligosaccharide ligands and for testing specimens of natural capsular polysaccharides and immune sera.

Key words

Streptococcus pneumoniae type 14 capsular polysaccharide synthetic oligosaccharide fragment oligosaccharide-BSA conjugate antigenic activity 


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  1. 1.
    Tatochenko, V. K. (2008) Zh. Detsk. Infekts., 2, 13–17.Google Scholar
  2. 2.
    Tatochenko, V. K. (2010) Zh. Mikrobiol., 5, 90–98.PubMedGoogle Scholar
  3. 3.
    Vishnyakova, L. A. (1993) Pulmonologiya, 3, 17–20.Google Scholar
  4. 4.
    O’Brien, K. L., Wolfson, L. J., Watt, J. P., Henkle, E., Deloria-Knoll, M., McCall, N., Lee, E., Mulholland, K., Levine, O. S., and Cherian, T. (2009) Lancet, 374, 893–902.PubMedCrossRefGoogle Scholar
  5. 5.
    Weinberger, D. M., Harboe, Z. B., Sanders, E. A., Ndiritu, M., Klugman, K. P., Ruckinger, S., Dagan, R., Adegbola, R., Cutts, F., Johnson, H. L., O’Brien, K. L., Scott, J. A., and Lipsitch, M. (2010) Clin. Infect. Dis., 51, 692–699.PubMedCrossRefGoogle Scholar
  6. 6.
    Melin, M., Trzcinski, K., Antonio, M., Meri, S., Adegbola, R., Kaijalainen, T., Kayhty, H., and Vakevainen, M. (2010) Infect. Immun., 78, 5252–5261.PubMedCrossRefGoogle Scholar
  7. 7.
    Hyams, C., Yuste, J., Bax, K., Camberlein, E., Weiser, J. N., and Brown, J. S. (2010) Infect. Immun., 78, 716–725.PubMedCrossRefGoogle Scholar
  8. 8.
    Kamerling, J. P. (2000) in Streptococcus Pneumoniae Molecular Biology and Mechanism of Disease (Tomasz, A., ed.) Mary Ann Libert, pp. 81–114.Google Scholar
  9. 9.
    Prevention of Pneumococcal Disease among Infants and Children — Use of 13-Valent Pneumococcal Conjugate Vaccine and 23-Valent Pneumococcal Polysaccharide Vaccine (2010) MMWR Recomm. Rep., 59(RR-11), 1–18.Google Scholar
  10. 10.
    Kozlov, P. S. (2010) MAK/MAX, 25.Google Scholar
  11. 11.
    Kozlov, P. S., Chagaryan, A. N., Kozlova, L. V., and Muraviev, A. A. (2011) Klin. Mikrobiol. Antimikrob. Khimioter., 13, 177–187.Google Scholar
  12. 12.
    Coffey, T. J., Berron, S., Daniels, M., Garcia-Leoni, M. E., Cercenado, E., Bouza, E., Fenoll, A., and Spratt, B. G. (1996) Microbiology, 142, 2747–2757.CrossRefGoogle Scholar
  13. 13.
    Rusen, I. D., and Fraser-Poberts, M. D. (1997) Ped. Infect. Dis., 16, 656–662.CrossRefGoogle Scholar
  14. 14.
    Jansen, W. T., and Snippe, H. (2004) Ind. J. Med. Res., 119(Suppl.), 7–12.Google Scholar
  15. 15.
    Reinter, R. R., Kaufhold, A., Kuhnemund, O., and Luttichen, R. (1994) Int. J. Med. Microbiol. Virol. Parasitol. Infect. Dis., 281, 481–490.Google Scholar
  16. 16.
    Hausdorf, W. P., Bryant, J., Paradiso, P. R., and Siber, G. R. (2000) Clin. Infect. Dis., 30, 100–131.CrossRefGoogle Scholar
  17. 17.
    Lindberg, B., Lönngren, J., and Powel, D. A. (1977) Carbohydr. Res., 58, 177–186.PubMedCrossRefGoogle Scholar
  18. 18.
    Safari, D., Dekker, H. A. T., and Joosten, A. F. (2008) Infect. Immun., 76, 4615–4623.PubMedCrossRefGoogle Scholar
  19. 19.
    Egorova, N. B., Kurbatova, E. A., Miroshnichenko, I. V., Mashilova, G. M., Gladus, M. A., Lazareva, E. S., Kiseleva, B. S., and Vaneeva, N. P. (1983) Zh. Mikrobiol., 2, 96–100.PubMedGoogle Scholar
  20. 20.
    Kochetkov, N. K., Nifant’ev, N. E., and Backinowsky, L. V. (1987) Tetrahedron, 43, 3109–3121.CrossRefGoogle Scholar
  21. 21.
    Nifant’ev, N. E., Backinowsky, L. V., and Kochetkov, N. K. (1988) Carbohydr. Res., 174, 61–72.CrossRefGoogle Scholar
  22. 22.
    Chernyak, A., Karavanov, A., Ogawa, Y., and Kovac, P. (2001) Carbohydr. Res., 330, 479–486.PubMedCrossRefGoogle Scholar
  23. 23.
    Hou, S.-J., Saksena, R., and Kovac, P. (2008) Carbohydr. Res., 343, 196–210.PubMedCrossRefGoogle Scholar
  24. 24.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.PubMedGoogle Scholar
  25. 25.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1951) Nature, 168, 167.PubMedCrossRefGoogle Scholar
  26. 26.
    Safari, D., Dekker, H. A. T., Joosten, J. A. F., Michalik, D., Carvalho de Souza, A., Adamo, R., Lahmann, M., Sundgren, A., Oscarson, S., Kamerling, J. P., and Snippe, H. (2008) Infect. Immun., 76, 4615–4623.PubMedCrossRefGoogle Scholar
  27. 27.
    Mammen, M., Choi, S.-K., and Whitesides, G. M. (1998) Angew. Chem. Int. Ed., 37, 2754–2794.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • E. A. Kurbatova
    • 1
  • D. S. Vorobiov
    • 1
  • I. B. Semenova
    • 1
  • E. V. Sukhova
    • 2
  • D. V. Yashunsky
    • 2
    • 3
  • Y. E. Tsvetkov
    • 2
  • N. E. Nifantiev
    • 2
  1. 1.Mechnikov Research Institute of Vaccines and SeraRussian Academy of Medical SciencesMoscowRussia
  2. 2.Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Orekhovich Research Institute of Biomedical ChemistryRussian Academy of Medical SciencesMoscowRussia

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