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Biochemistry (Moscow)

, Volume 78, Issue 7, pp 710–720 | Cite as

Mass spectrometry of glycans

  • Liang Han
  • Catherine E. CostelloEmail author
Review

Abstract

Powerful new strategies based on mass spectrometry are revolutionizing the structural analysis and profiling of glycans and glycoconjugates. We survey here the major biosynthetic pathways that underlie the biological diversity in glycobiology, with emphasis on glycoproteins, and the approaches that can be used to address the resulting heterogeneity. Included among these are derivatizations, on- and off-line chromatography, electrospray and matrix-assisted laser desorption/ionization, and a variety of dissociation methods, the recently introduced electron-based techniques being of particular interest.

Key words

mass spectrometry MS/MS glycobiology structural determinations biosynthesis chromatography derivatives 

Abbreviations

CID

collision-induced dissociation

ECD

electron capture dissociation

EDD

electron detachment dissociation

EED

electronic excitation dissociation

ER

endoplasmic reticulum

ESI

electrospray ionization

ETD

electron transfer dissociation

FT-ICR

Fourier transform ion cyclotron resonance

GC/MS

gas chromatography coupled to mass spectrometry

HPLC

high-performance liquid chromatography

IM-MS

ion mobility-mass spectrometry

LC/MS

liquid chromatography/mass spectrometry

MALDI

matrix-assisted laser desorption/ionization

MALDI-TOF

matrix-assisted laser desorption/ionization-time of flight

MS

mass spectrometry

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Supplementary material

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Center for Biomedical Mass SpectrometryBoston University School of MedicineBostonUSA

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