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Biochemistry (Moscow)

, Volume 78, Issue 7, pp 697–709 | Cite as

High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate analysis of glycoproteins

  • J. S. RohrerEmail author
  • L. Basumallick
  • D. Hurum
Review

Abstract

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is an established technique for the carbohydrate analysis of glycoproteins. HPAE-PAD is routinely used for determinations of monosaccharide, sialic acid, mannose-6-phosphate (M-6-P), and oligosaccharide contents of a glycoprotein. This is true for both the initial investigation of a glycoprotein and routine assays of recombinant therapeutic glycoproteins. This contribution reviews the fundamentals of HPAE-PAD, recent technological improvements, and advances in the last ten years in its application to carbohydrate analysis of glycoproteins. The application areas reviewed include monosaccharide determinations, sialic acid determinations, M-6-P determinations, sugar alcohol determinations, analysis of polysialic acids, neutral and charged oligosaccharide analysis, following glycosidase and glycosyltransferase reactions, and coupling HPAE-PAD to mass spectrometry (MS).

Key words

monosaccharide sialic acid oligosaccharide glycoprotein carbohydrate mannose-6-phosphate HPAE-PAD 

Abbreviations

DMB

1,2-diamino-4,5-methylenedioxybenzene

DP

degree of polymerization

HPAE-PAD

high-performance anion-exchange chromatography with pulsed amperometric detection

IC

ion chromatography

LC

liquid chromatography

MALDI-TOF-MS

matrix-assisted laser desorption time-of-flight mass spectrometry

M-6-P

mannose-6-phosphate

MS

mass spectrometry

Neu5Ac

N-acetylneuraminic acid

Neu5Gc

N-glycolylneuraminic acid

PE

polyester

PEEK

polyether ether ketone polymer

PTFE

polytetrafluoroethylene

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References

  1. 1.
    Rocklin, R. D., and Pohl, C. A. (1983) J. Liq. Chromatogr., 6, 1577–1590.CrossRefGoogle Scholar
  2. 2.
    Hardy, M. R., Townsend, R. R., and Lee, Y. C. (1988) Anal. Biochem., 170, 54–62.PubMedCrossRefGoogle Scholar
  3. 3.
    Chen, L-M., Yet, M-G., and Shao, M.-C. (1988) FASEB J., 2, 2819–2824.PubMedGoogle Scholar
  4. 4.
    Spellman, M. W. (1990) Anal. Chem., 62, 1714–1722.PubMedCrossRefGoogle Scholar
  5. 5.
    Lee, Y. C. (1990) Anal. Biochem., 189, 151–162.PubMedCrossRefGoogle Scholar
  6. 6.
    Lee, Y. C. (1996) J. Chromatogr. A, 720, 137–149.CrossRefGoogle Scholar
  7. 7.
    Cataldi, R. I., Campa, C., and De Benedette, G. E. (2000) Fresenius J. Anal. Chem., 368, 739–758.PubMedCrossRefGoogle Scholar
  8. 8.
    Rohrer, J. S. (2000) Anal. Biochem., 283, 3–9.PubMedCrossRefGoogle Scholar
  9. 9.
    Hardy, M. R., and Rohrer, J. S. (2007) in Comprehensive Glycoscience (Kamerling, J. P., ed.) Vol. 2, Elsevier, Netherlands, pp. 303–327.Google Scholar
  10. 10.
    Higgins, E. (2010) Glycoconj. J., 27, 211–225.PubMedCrossRefGoogle Scholar
  11. 11.
    Behan, J. L., and Smith, K. D. (2011) Biomed. Chromatogr., 25, 39–46.PubMedCrossRefGoogle Scholar
  12. 12.
    Rocklin, R. D., Clarke, A. P., and Weitzhandler, M. (1998) Anal. Chem., 70, 1496–1501.CrossRefGoogle Scholar
  13. 13.
    Rohrer, J. S. (1998) Thermo Fisher Scientific Dionex Technical Note 21 (http://www.dionex.com/en-us/web-docs/5050-TN21-LPN034889-03.pdf).Google Scholar
  14. 14.
    Hurum, D., Christenson, T., Perati, P., Basumallick, L., and Rohrer, J. (2011) Thermo Fisher Scientific Dionex Technical Note 110 (http://www.dionex.com/en-us/webdocs/111176-TN110-IC-Carb-HPAEPADdisposAuPTFE-12Oct2011-LPN2952-R2.pdf).Google Scholar
  15. 15.
    Rohrer, J. S. (2007) Thermo Fisher Scientific Dionex Technical Note 71 (http://www.dionex.com/en-us/webdocs/58087-TN71-Eluent-Prep-HPAE-PAD-16Sept2009-LPN1932-02.pdf).Google Scholar
  16. 16.
    Kotnik, D., Novic, M., LaCourse, W. R., and Pihlar, B. (2011) J. Electroanal. Chem., 683, 30–35.Google Scholar
  17. 17.
    Kotnik, D., Novic, M., Novic, M., Pihlar, B., and Neubauer, D. (2012) Poster at the Int. Ion Chromatography Symp., Berlin, Germany.Google Scholar
  18. 18.
    Hurum, D. C., and Rohrer, J. S. (2011) Anal. Biochem., 419, 67–69.PubMedCrossRefGoogle Scholar
  19. 19.
    Cheng, J. (2012) Presentation at the 2012 Pittsburgh Conference, Orlando, FL USA.Google Scholar
  20. 20.
    Rohrer, J. S. (2012) in Applications of Ion Chromatography in the Analysis of Pharmaceutical and Biological Products (Bhattacharyya, L., and Rohrer, J. S., eds.) John Wiley and Sons Inc., Hoboken, New Jersey, pp. 339–350.Google Scholar
  21. 21.
    Basumallick, L., and Rohrer, J. (2012) Thermo Fisher Scientific Dionex Technical Note 40 (http://www.dionex.com/en-us/webdocs/5052-TN40-IC-Glycoprotein-Monosaccharide-23May2012-LPN1632-01.pdf).Google Scholar
  22. 22.
    Zhang, Z., Khan, N. M., Nunez, K. M., Chess, E. K., and Szabo, C. M. (2012) Anal. Chem., 84, 4104–4110.PubMedCrossRefGoogle Scholar
  23. 23.
    Herrmann, A., Konig, S., Lechtenberg, M., Sehlbach, M., Vakhrushev, S. Y., Peter-Katalinic, J., and Hensel, A. (2012) Glycobiology, 22, 1424–1439.PubMedCrossRefGoogle Scholar
  24. 24.
    Bergfeld, A. K., Pearce, O. M. T., Diaz, S. L., Pham, T., and Varki, A. (2012) J. Biol. Chem., 287, 28865–28881.PubMedCrossRefGoogle Scholar
  25. 25.
    Everest-Dass, A. V., Jin, D., Thaysen-Andersen, M., Nevalainen, H., Kolarich, D., and Packer, N. H. (2012) Glycobiology, 22, 1465–1479.PubMedCrossRefGoogle Scholar
  26. 26.
    Adamo, M., Qui, D., Dick, L. W., Jr., Zeng, M., Lee, A-H., and Cheng, K-C. (2009) J. Pharm. Biomed. Anal., 49, 181–192.PubMedCrossRefGoogle Scholar
  27. 27.
    Machado, E., Kandzia, S., Carilho, R., Altevogt, P., Conradt, H. S., and Costa, J. (2011) Glycobiology, 21, 376–386.PubMedCrossRefGoogle Scholar
  28. 28.
    Yu, T., Guo, C., Wang, J., Hao, P., Sui, S., Chen, X., Zhang, R., Wang, P., Yu, G., Zhang, L., Dai, Y., and Li, N. (2011) Glycobiology, 21, 206–224.PubMedCrossRefGoogle Scholar
  29. 29.
    Clarke, A. J., Sarabia, V., Keenleyside, W., MacLachlan, P. R., and Whitfield, C. (1991) Anal. Biochem., 199, 68–74.PubMedCrossRefGoogle Scholar
  30. 30.
    Harazono, A., Kobayashi, T., Kawasaki, N., Itoh, S., Tada, M., Hashii, N., Ishii, A., Arato, T., Yanagihara, S., Yagi, Y., Koga, A., Tsuda, Y., Kimura, M., Sakita, M., Kitamura, S., Yamaguchi, H., Mimura, H., Murata, Y., Hamazume, Y., Sato, T., Natsuka, S., Kakehi, K., Kinoshita, M., Watanabe, S., and Yamaguchi, T. (2011) Biologicals, 39, 171–180.PubMedCrossRefGoogle Scholar
  31. 31.
    Padler-Karavani, V., Yu, H., Cao, H., Chokhawala, H., Karp, F., Varki, N., Chen, X., and Varki, A. (2008) Glycobiology, 18, 818–830.PubMedCrossRefGoogle Scholar
  32. 32.
    Raju, T. S., Briggs, J. B., Borge, S. M., and Jones, A. J. (2000) Glycobiology, 10, 477–486.PubMedCrossRefGoogle Scholar
  33. 33.
    Baker, K. N., Rendall, M. H., Hills, A. E., Hoare, M., Freedman, R. B., and James, D. C. (2001) Biotechnol. Bioeng., 73, 188–202.PubMedCrossRefGoogle Scholar
  34. 34.
    Manzi, A. E., Diaz, S., and Varki, A. (1990) Anal. Biochem., 188, 20–32.PubMedCrossRefGoogle Scholar
  35. 35.
    Hara, S., Yamaguchi, M., Takemori, Y., Nakamura, M., and Ohkura, Y. (1986) J. Chromatogr. B, 377, 111–119.CrossRefGoogle Scholar
  36. 36.
    Hara, S., Yamaguchi, M., Takemori, Y., Furuhata, K., Ogura, H., and Nakamura, M. (1989) Anal. Biochem., 179, 162–166.PubMedCrossRefGoogle Scholar
  37. 37.
    Hurum, D. C., and Rohrer, J. S. (2012) Gen. Eng. Biotech. News, 32, 18–19.CrossRefGoogle Scholar
  38. 38.
    Suriano, R., Ghosh, S. K., Chaudhuri, D., Mittelman, A., Banerjee, A., and Tiwari, R. K. (2009) Glycobiology, 19, 1427–1435.PubMedCrossRefGoogle Scholar
  39. 39.
    Suzuki, N., Su, T-H., Wu, S-W., Yamaloto, K., Khoo, K-W., and Lee, Y. C. (2009) Glycobiology, 19, 693–706.PubMedCrossRefGoogle Scholar
  40. 40.
    Zhang, Y., Inoue, Y., Inoue, S., and Lee, Y. C. (1997) Anal. Biochem., 250, 245–251.PubMedCrossRefGoogle Scholar
  41. 41.
    Inoue, Y., and Inoue, S. (2001) Glycobiology, 11, 759–767.PubMedCrossRefGoogle Scholar
  42. 42.
    Lin, S.-L., Inoue, Y., and Inoue, S. (1999) Glycobiology, 9, 807–814.PubMedCrossRefGoogle Scholar
  43. 43.
    Vionnet, J., and Vann, W. F. (2007) Glycobiology, 17, 735–743.PubMedCrossRefGoogle Scholar
  44. 44.
    Nakata, D., and Troy, F. A., 2nd (2005) J. Biol. Chem., 280, 38305–38316.PubMedCrossRefGoogle Scholar
  45. 45.
    Wong, K. S., and Jane, J. (1995) J. Liq. Chromatogr., 18, 63–80.CrossRefGoogle Scholar
  46. 46.
    Basumallick, L., and Rohrer, J. (2012) Thermo Fisher Scientific Dionex Application Note 1013 (http://www.dionex.com/en-us/webdocs/113699-AN1013-IC-Polysialic-Acid-Polymers-08Aug2012-AN70124-E-R2.pdf).Google Scholar
  47. 47.
    Chen, L-M., Yet, M-G., and Shao, M.-C. (1988) FASEB J., 2, 2819–2824.PubMedGoogle Scholar
  48. 48.
    Townsend, R. R., Hardy, M. R., Hindsgaul, O., and Lee, Y. C. (1988) Anal. Biochem., 174, 459–470.PubMedCrossRefGoogle Scholar
  49. 49.
    Rohrer, J. S. (1995) Glycobiology, 5, 359–360.PubMedCrossRefGoogle Scholar
  50. 50.
    Perati, P., and Rohrer, J. (2010) Thermo Fisher Scientific Dionex Application Update 176 (http://www.dionex.com/en-us/webdocs/88108-AU176-IC-PNGaseF-HPAEPAD-07Sep2010-LPN2576-R2.pdf).Google Scholar
  51. 51.
    Hayase, T., Sheykhanazari, M., Bhavanandan, V. P., Savage, A. V., and Lee, Y. C. (1993) Anal. Biochem., 211, 72–80.PubMedCrossRefGoogle Scholar
  52. 52.
    Grey, C., Edebrink, P., Krook, M., and Jacobssin, S. P. (2009) J. Chromatogr. B, 877, 1827–1832.CrossRefGoogle Scholar
  53. 53.
    Zheng, T., Rohrer, J., and Rao, S. (2010) Genet. Eng. News, 30, 42–43.Google Scholar
  54. 54.
    Zheng, T., Rao, S., Rohrer, J., and Pohl, C. (2012) in Antibody-Mediated Drug Delivery Systems (Pathak, Y., and Benita, S., eds.) John Wiley and Sons Inc., Hoboken, New Jersey, pp. 145–167.Google Scholar
  55. 55.
    Van Berkel, P. H. C., Gerritsen, J., Perdok, G., Valbjorn, J., Vink, T., van de Winkel, J. G. J., and Parren, P. W. H. I. (2009) Biotechnol. Prog., 25, 244–251.PubMedCrossRefGoogle Scholar
  56. 56.
    Machado, E., Kandzia, S., Carilho, R., Altevogt, P., Conradt, H. S., and Costa, J. (2011) Glycobiology, 21, 376–386.PubMedCrossRefGoogle Scholar
  57. 57.
    Simpson, R. C., Fenselau, C. C., Hardy, M. R., Townsend, R. R., and Lee, Y. C. (1990) Anal. Chem., 62, 248–252.PubMedCrossRefGoogle Scholar
  58. 58.
    Conboy, J. J., and Henion, J. (1992) Biol. Mass Spectr., 21, 397–407.CrossRefGoogle Scholar
  59. 59.
    Thayer, J., Rohrer, J. S., Avdalovic, N., and Gearing, R. P. (1998) Anal. Biochem., 256, 207–216.PubMedCrossRefGoogle Scholar
  60. 60.
    Bruggink, C., Maurer, R., Herrman, H., Cavalli, S., and Hoefler, F. (2005) J. Chromatogr. A, 1085, 104–109.PubMedCrossRefGoogle Scholar
  61. 61.
    Bruggink, C., Wuhrer, M., Koeleman, C. A. M., Barreto, V., Liu, Y., Pohl, C., Ingendoh, A., Hokke, C. H., and Deelder, A. (2005) J. Chromatogr. B, 829, 136–143.CrossRefGoogle Scholar
  62. 62.
    Bruggink, C., Poorthuis, B. J. H. M., Deelder, A. M., and Wuhrer, M. (2012) Anal. Bioanal. Chem., 403, 1671–1683.PubMedCrossRefGoogle Scholar
  63. 63.
    Chataigne, G., Couderc, F., and Poinsot, V. (2008) J. Chromatogr. A, 1185, 241–250.PubMedCrossRefGoogle Scholar
  64. 64.
    Boschker, H. T. S., Moerdijk-Peertvliet, T. C. W., van Breugel, P., Houtekamer, M., and Middelburg, J. J. (2008) Rapid Commun. Mass Spectrom., 22, 3902–3908.PubMedCrossRefGoogle Scholar
  65. 65.
    Sartor, P. A., Agusti, R., Leguizamon, M. S., Campetella, O., and de Lederkremer, R. M. (2010) Glycobiology, 20, 982–990.PubMedCrossRefGoogle Scholar
  66. 66.
    Nguyen, K., van Die, I., Grundahl, K. M., Kawar, Z. S., and Cummings, R. D. (2007) Glycobiology, 17, 586–599.PubMedCrossRefGoogle Scholar
  67. 67.
    Zhang, P., and Hu, H. (2012) Glycobiology, 22, 235–247.PubMedCrossRefGoogle Scholar
  68. 68.
    Heise, N., Singh, D., van der Wei, H., Sassi, S. O., Johnson, J. M., Feasly, C. L., Koeller, C. M., Previato, J. O., Mendoca-Previato, L., and West, C. M. (2009) Glycobiology, 19, 918–933.PubMedCrossRefGoogle Scholar
  69. 69.
    Lee, K., Jin, X., Zhang, K., Copertino, L., Andrews, L., Baker-Malcolm, J., Geagan, L., Qiu, H., Seiger, K., Barngrover, D., McPherson, J. M., and Edmunds, T. (2003) Glycobiology, 13, 305–313.PubMedCrossRefGoogle Scholar
  70. 70.
    Van Patten, S. M., Hughes, H., Huff, M. R., Piepenhagen, P. A., Waire, J., Qiu, H., Ganesa, C., Reczek, D., Ward, P. V., Kutzko, J. P., and Edmunds, T. (2007) Glycobiology, 17, 467–478.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Thermo Fisher ScientificSunnyvaleUSA

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