Advertisement

Biochemistry (Moscow)

, Volume 78, Issue 4, pp 424–430 | Cite as

Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414

  • Huan Qi
  • Fali Bai
  • Aihua LiuEmail author
Article

Abstract

By combining anion-exchange chromatography with gel filtration, an effective method for purification of wild-type xyloglucanase and five other cellulolytic enzymes from strain QM9414 of Trichoderma reesei was established. Characterization by enzyme activity assay, SDS-PAGE, and mass spectrometry identified the purified proteins as cellobiohydrolases I and II, endoglucanases I and II, a xyloglucanase, and β-xylosidase, of which the xyloglucanase was purified for the first time from the mutant strain QM9414. This method holds great promise to study the mechanism of cellulolytic enzymes, to investigate the synergistic action between cellulase and other cellulolytic enzymes, and to better exploit enzyme preparations for degradation of lignocellulose.

Key words

xyloglucanase cellulolytic enzymes purification Trichoderma reesei QM9414 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Jarvis, M. (2003) Nature, 426, 611–612.PubMedCrossRefGoogle Scholar
  2. 2.
    Huang, R. L., Su, R. X., Qi, W., and He, Z. M. (2011) Bioenerg. Res., 4, 225–245.CrossRefGoogle Scholar
  3. 3.
    Willis, J. D., Oppert, C., and Jurat-Fuentes, J. L. (2010) Insect. Sci., 17, 184–198.CrossRefGoogle Scholar
  4. 4.
    Lantz, S. E., Goedegebuur, F., Hommes, R., Kaper, T., Kelemen, B. R., Mitchinson, C., Wallace, L., Stahlberg, J., and Larenas, E. A. (2010) Biotechnol. Biofuels, 3, 20.PubMedCrossRefGoogle Scholar
  5. 5.
    Zhou, L., Wu, X., Lan, L., and Liu, J. (2010) Biotechnol. Lett., 32, 67–72.PubMedCrossRefGoogle Scholar
  6. 6.
    Montenecourt, B. S., and Eveleigh, D. E. (1977) Appl. Environ. Microbiol., 34, 777–782.PubMedGoogle Scholar
  7. 7.
    Zhang, J., Zhong, Y., Zhao, X., and Wang, T. (2010) Bioresour. Technol., 101, 9815–9818.PubMedCrossRefGoogle Scholar
  8. 8.
    Gao, D., Chundawat, S. P. S., Krishnan, C., Balan, V., and Dale, B. E. (2010) Bioresour. Technol., 101, 2770–2781.PubMedCrossRefGoogle Scholar
  9. 9.
    Wang, F. C., Li, F., Chen, G. J., and Liu, W. F. (2009) Microbiol. Res., 164, 650–657.PubMedCrossRefGoogle Scholar
  10. 10.
    Tomaz, C. T., and Queiroz, J. A. (2004) Biotechnol. Lett., 26, 223–227.PubMedCrossRefGoogle Scholar
  11. 11.
    Homma, T., Fujii, M., and Titus, U. (1992) J. Chem. Eng. Jpn., 25, 288–293.CrossRefGoogle Scholar
  12. 12.
    Schmid, G., and Wandrey, C. (1987) Biotechnol. Bioeng., 30, 571–585.PubMedCrossRefGoogle Scholar
  13. 13.
    Bhikhabhai, R., Johansson, G., and Pettersson, G. (1984) J. Appl. Biochem., 6, 336–345.PubMedGoogle Scholar
  14. 14.
    Duenas, M. J., and Estrada, P. (1999) Biocatal. Biotransform., 17, 139–161.CrossRefGoogle Scholar
  15. 15.
    Deshpande, P., Nair, S., and Khedkar, S. (2009) Appl. Biochem. Biotech., 158, 552–560.CrossRefGoogle Scholar
  16. 16.
    Bradford, M. M. (1976) Anal. Biochem., 72, 248–254.PubMedCrossRefGoogle Scholar
  17. 17.
    Shevchenko, A., Wilm, M., Vorm, O., Jensen, O. N., Podtelejnikov, A. V., Neubauer, G., Mortensen, P., and Mann, M. (1996) Biochem. Soc. Trans., 24, 893–896.PubMedGoogle Scholar
  18. 18.
    Hu, R. F., Lin, L., Liu, T. J., Ouyang, P., He, B. H., and Liu, S. J. (2008) J. Biobased Mater. Bio., 2, 156–161.CrossRefGoogle Scholar
  19. 19.
    Subramaniyan, S., Prema, P., and Ramakrishna, S. V. (1997) J. Basic Microb., 37, 431–437.CrossRefGoogle Scholar
  20. 20.
    Hahn-Hagerdal, B., Galbe, M., Gorwa-Grauslund, M. F., Liden, G., and Zacchi, G. (2006) Trends Biotechnol., 24, 549–556.PubMedCrossRefGoogle Scholar
  21. 21.
    Shoemaker, S., Watt, K., Tsitovsky, G., and Cox, R. (1983) Nat. Biotechnol., 1, 687–690.CrossRefGoogle Scholar
  22. 22.
    Gao, L., Wang, F., Gao, F., Wang, L., Zhao, J., and Qu, Y. (2011) Bioresour. Technol., 102, 8339–8342.PubMedCrossRefGoogle Scholar
  23. 23.
    Foreman, P. K., Brown, D., Dankmeyer, L., Dean, R., Diener, S., Dunn-Coleman, N. S., Goedegebuur, F., Houfek, T. D., England, G. J., Kelley, A. S., Meerman, H. J., Mitchell, T., Mitchinson, C., Olivares, H. A., Teunissen, P. J., Yao, J., and Ward, M. (2003) J. Biol. Chem., 278, 31988–31997.PubMedCrossRefGoogle Scholar
  24. 24.
    Zhang, J., Tuomainen, P., Siika-Aho, M., and Viikari, L. (2011) Bioresour. Technol., 102, 9090–9095.PubMedCrossRefGoogle Scholar
  25. 25.
    Alvira, P., Negro, M. J., and Ballesteros, M. (2011) Bioresour. Technol., 102, 4552–4558.PubMedCrossRefGoogle Scholar
  26. 26.
    Beukes, N., and Pletschke, B. I. (2010) Bioresour. Technol., 101, 4472–4478.PubMedCrossRefGoogle Scholar
  27. 27.
    Qing, Q., Yang, B., and Wyman, C. E. (2010) Bioresour. Technol., 101, 9624–9630.PubMedCrossRefGoogle Scholar
  28. 28.
    Burgess, A. W., Lloyd, C. J., and Nice, E. C. (1983) EMBO J., 2, 2065–2069.PubMedGoogle Scholar
  29. 29.
    Beldman, G., Searle-Van Leeuwen, M. F., Rombouts, F. M., and Voragen, F. G. (1985) Eur. J. Biochem., 146, 301–308.PubMedCrossRefGoogle Scholar
  30. 30.
    Hakansson, U., Fagerstam, L. G., Pettersson, L. G., and Andersson, L. (1979) Biochem. J., 179, 141–149.PubMedGoogle Scholar
  31. 31.
    Gusakov, A. V., Grishutin, S. G., Markov, A. V., Ustinov, B. B., Semenova, M. V., and Sinitsyn, A. P. (2004) BBA-Gen Subjects, 1674, 268–281.CrossRefGoogle Scholar
  32. 32.
    Bukhtojarov, F. E., Ustinov, B. B., Salanovich, T. N., Antonov, A. I., Gusakov, A. V., Okunev, O. N., and Sinitsyn, A. P. (2004) Biochemistry (Moscow), 69, 542–551.CrossRefGoogle Scholar
  33. 33.
    Nikupaavola, M. L., Lappalainen, A., Enari, T. M., and Nummi, M. (1985) Biochem. J., 231, 75–81.Google Scholar
  34. 34.
    Golubev, A. M., Brandao Neto, J. R., Eneyskaya, E. V., Kulminskaya, A. V., Kerzhner, M. A., Neustroev, K. N., and Polikarpov, I. (2000) Acta Crystallogr. D. Biol. Crystallogr., 56, 1058–1060.PubMedCrossRefGoogle Scholar
  35. 35.
    Poutanen, K., and Puls, J. (1988) Appl. Microbiol. Biot., 28, 425–432.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Qingdao Institute of Bioenergy and Bioprocess Technology and Key Laboratory of BioenergyChinese Academy of SciencesQingdaoChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

Personalised recommendations