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Biochemistry (Moscow)

, Volume 78, Issue 4, pp 418–423 | Cite as

Targeted delivery of siRNA to differentiated murine myotubes in culture by a conjugate of cationic oligopeptide with FS2 venom

  • V. A. Furalyov
  • I. V. Kravchenko
  • V. O. Popov
Article

Abstract

A conjugate of the ligand of FS2 venom dihydropyridine receptors with a cationic arginine-containing oligopeptide was synthesized. It was found that the conjugate provides siRNA delivery to murine myotubes differentiated in vitro. The effect of RNA interference with the use of siRNA complexes with the conjugate was observed when siRNA concentrations were an order of magnitude lower than those used in the case of siRNA complexes with a non-conjugated oligopeptide.

Key words

siRNA RNA interference myostatin myotubes 

Abbreviations

FCS

fetal calf serum

GAPD

glyceraldehyde phosphate dehydrogenase

ME

β-mercaptoethanol

PBS

phosphate-buffered saline

PCR

polymerase chain reaction

siRNA

small interfering RNA

sulfoSPDP

sulfosuccinimidyl-pyridyldithiopropionamidohexanoate

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • V. A. Furalyov
    • 1
  • I. V. Kravchenko
    • 1
  • V. O. Popov
    • 1
  1. 1.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia

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