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Biochemistry (Moscow)

, Volume 78, Issue 2, pp 185–193 | Cite as

Involvement of Toll-like receptor 4 and Fc receptors gamma in human neutrophil priming by endotoxins from Escherichia coli

  • D. S. KabanovEmail author
  • I. R. Prokhorenko
Article

Abstract

By using the fMLP-induced respiratory burst approach, the involvement of Toll-like receptor 4 (TLR4) in human neutrophil priming by S- or Re-glycoforms of endotoxin from Escherichia coli has been elucidated. The priming effect of Re-glycoform is more pronounced than that of the S-glycoform. Unexpectedly, fMLP-triggered generation of reactive oxygen species (ROS) by endotoxin primed neutrophils was amplified by preincubation of the cells with anti-TLR4 (HTA125) antibodies or with isotype-matched immunoglobulin IgG2a. The most significant finding of our study is that neutrophils exposed to anti-TLR4 antibodies retain their ability to distinguish between S- or Re-glycoforms being primed, respectively. Moreover, differentiated effect of HTA125 antibodies on functional responses of neutrophils during their priming and fMLP stimulation was revealed. Taking these results into consideration, it is reasonable to assume that there is a contribution of Fcγ receptors to fMLP-induced ROS generation by neutrophils preincubated with HTA125 or IgG2a and primed by endotoxins.

Key words

lipopolysaccharide (endotoxin) Toll-like receptor 4 receptor cluster Fcγ receptors IgG2a anti-human TLR4 antibody reactive oxygen species 

Abbreviations

AA

arachidonic acid

Akt

serine/threonine protein kinase B (PKB)

CD

cluster of differentiation

DAG

diacyl-glycerol

ERK

extracellularly-regulated kinase (MAPK, p38)

fMLP

N-formyl-methionyl-leucyl-phenylalanine

IKK

inhibitory protein IκB kinase

IL

interleukin

IRAK

interleukin-1 receptor-associated kinase

ITAM

immunoreceptor tyrosine-based activation motif

ITIM

immunoreceptor tyrosine-based inhibitory motif

LBP

LPS-binding protein

LOS

lipooligosaccharide

LPS

lipopolysaccharide

LYN

tyrosine kinase

Mal

MyD88-like adaptor molecule

MD-2

myeloid differentiation factor 2, a secreted glycoprotein

MEK

mitogen-activated ERK-activating kinase (MAPKK)

MKK3/6

mitogen-activated kinase kinase 3/6

MyD88

myeloid factor of differentiation

NADPH

oxidase membrane components: flavocytochrome b 558 [gp91phox, p22phox] and Rap1A, a protein binding guanosine triphosphate

NADPH

oxidase cytoplasmic components: p40phox, p47phox, p67phox

NF-κB

transcription factor κB

PAK

p21-activated kinase

PI3Kγ

phosphatidylinositol-3-kinase γ

PIP2

phosphatidylinositol-4,5-biphosphate

PIP3

phosphatidylinositol-3,4,5-triphosphate

PKC

protein kinase C

PL

phospholipid

PLCβ2

phospholipase Cβ2

PLCγ

phospholipase Cγ

Rac, Rho, Ras and CDC42

intracellular signaling proteins hydrolyzing guanosine triphosphate

Raf

serine/threonine protein kinases

ROS

reactive oxygen species

sCD14

soluble form of CD14 not associated with cell membranes

siRNA

small interference RNA

Src

tyrosine protein kinase

SYK

tyrosine kinase

TAB

adaptor molecules

TAK1

TGFβ-activated kinase

TGFβ

transforming growth factor β

TIR

Toll/interleukin-1 receptor homology (TLR4 receptor domain homologous to interleukin-1 receptor domain)

TLR4

Toll-like receptor 4

TNF-α

tumor necrosis factor α

TRAF6

TNF-α receptor-associated factor

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© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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