Biochemistry (Moscow)

, Volume 78, Issue 2, pp 185–193 | Cite as

Involvement of Toll-like receptor 4 and Fc receptors gamma in human neutrophil priming by endotoxins from Escherichia coli

  • D. S. KabanovEmail author
  • I. R. Prokhorenko


By using the fMLP-induced respiratory burst approach, the involvement of Toll-like receptor 4 (TLR4) in human neutrophil priming by S- or Re-glycoforms of endotoxin from Escherichia coli has been elucidated. The priming effect of Re-glycoform is more pronounced than that of the S-glycoform. Unexpectedly, fMLP-triggered generation of reactive oxygen species (ROS) by endotoxin primed neutrophils was amplified by preincubation of the cells with anti-TLR4 (HTA125) antibodies or with isotype-matched immunoglobulin IgG2a. The most significant finding of our study is that neutrophils exposed to anti-TLR4 antibodies retain their ability to distinguish between S- or Re-glycoforms being primed, respectively. Moreover, differentiated effect of HTA125 antibodies on functional responses of neutrophils during their priming and fMLP stimulation was revealed. Taking these results into consideration, it is reasonable to assume that there is a contribution of Fcγ receptors to fMLP-induced ROS generation by neutrophils preincubated with HTA125 or IgG2a and primed by endotoxins.

Key words

lipopolysaccharide (endotoxin) Toll-like receptor 4 receptor cluster Fcγ receptors IgG2a anti-human TLR4 antibody reactive oxygen species 



arachidonic acid


serine/threonine protein kinase B (PKB)


cluster of differentiation




extracellularly-regulated kinase (MAPK, p38)




inhibitory protein IκB kinase




interleukin-1 receptor-associated kinase


immunoreceptor tyrosine-based activation motif


immunoreceptor tyrosine-based inhibitory motif


LPS-binding protein






tyrosine kinase


MyD88-like adaptor molecule


myeloid differentiation factor 2, a secreted glycoprotein


mitogen-activated ERK-activating kinase (MAPKK)


mitogen-activated kinase kinase 3/6


myeloid factor of differentiation


oxidase membrane components: flavocytochrome b 558 [gp91phox, p22phox] and Rap1A, a protein binding guanosine triphosphate


oxidase cytoplasmic components: p40phox, p47phox, p67phox


transcription factor κB


p21-activated kinase


phosphatidylinositol-3-kinase γ






protein kinase C




phospholipase Cβ2


phospholipase Cγ

Rac, Rho, Ras and CDC42

intracellular signaling proteins hydrolyzing guanosine triphosphate


serine/threonine protein kinases


reactive oxygen species


soluble form of CD14 not associated with cell membranes


small interference RNA


tyrosine protein kinase


tyrosine kinase


adaptor molecules


TGFβ-activated kinase


transforming growth factor β


Toll/interleukin-1 receptor homology (TLR4 receptor domain homologous to interleukin-1 receptor domain)


Toll-like receptor 4


tumor necrosis factor α


TNF-α receptor-associated factor


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© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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