Biochemistry (Moscow)

, Volume 78, Issue 2, pp 157–165 | Cite as

Cap-independent translation initiation of Apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5′ untranslated region

  • D. E. Andreev
  • S. E. Dmitriev
  • I. M. Terenin
  • I. N. Shatsky
Accelerated Publication

Abstract

We have earlier shown that the 5′-untranslated region (5′ UTR) of the mRNA coding for activation factor of apoptotic peptidase 1 (Apaf-1) can direct translation in vivo by strictly 5′ end-dependent way even in the absence of m7G-cap. Dependence of translational efficiency on the cap availability for this mRNA turned out to be relatively low. In this study we demonstrate that this surprising phenomenon is determined the 5′-proximal part (domains I and II) of highly structured Apaf-1 5′ UTR. Remarkably, domain II by itself was able to reduce dependence of the mRNA on the cap on its transferring to a short 5′ UTR derived from a standard vector. We suggest that the low cap-dependence inherent to some cellular mRNAs may have an important physiological significance under those stress conditions when the function of cap-binding factor eIF4E is impaired.

Key words

protein biosynthesis translational control cellular IRES-elements cap-independent translation Apaf-1 mRNA 

Abbreviations

Apaf-1

apoptotic peptidase activating factor 1

CITEs

cap-independent translational enhancers

eIF

eukaryotic initiation factor

IRES

internal ribosome entry site

5′ UTR

5′-untranslated region

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • D. E. Andreev
    • 1
  • S. E. Dmitriev
    • 1
  • I. M. Terenin
    • 1
  • I. N. Shatsky
    • 1
  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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