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Biochemistry (Moscow)

, Volume 78, Issue 1, pp 102–110 | Cite as

Human gastrin-releasing peptide triggers growth of HepG2 cells through blocking endoplasmic reticulum stress-mediated apoptosis

  • Xinqiu Li
  • Litang Zhang
  • Xianzhu Ke
  • Yuming WangEmail author
Article

Abstract

Gastrin-releasing peptide (GRP) is a kind of neural peptide that plays an important role in the growth of various human cancer cells. However, very little is known about the relationship between GRP and apoptosis in human hepatocellular carcinoma cells. This study investigated the influences of GRP on apoptosis, as well as the mechanism that triggers HepG2 growth. The effects of GRP on cell proliferation were examined by analysis of lactate dehydrogenase. The GRP, caspase 12, and CHOP protein were detected in HepG2 and HL-7702 cells by Western blot, and endoplasmic reticulum (ER) stress-related mRNA transcription was detected by reverse transcription polymerase chain reaction. To explore the specific pathway by which GRP induces the cell growth, we investigated the apoptosis-related pathway. The expression of GRP in HL-7702 cells inhibited tunicamycin triggered ER stress-associated XBP1, ATF4, and TRAF2 mRNA transcription. Three main ER stress-unfolded protein response pathways proteins, including spliced XBP1, cleaved ATF6, IRE1-α, PERK, and eIF2-α, were increased significantly. Furthermore, the cleaved caspase 12 activation was blocked and CHOP expression was inhibited when GRP was expressed either in HepG2 or HL-7702 cells. In conclusion, GRP triggers the growth of HepG2 cells through blocking the ER stress-mediated pathway.

Key words

gastrin-releasing peptide growth endoplasmic reticulum stress apoptosis Published in Russian in Biokhimiya, 2013, Vol. 78, No. 1, pp. 131–142. 

Abbreviations

ER

endoplasmic reticulum

GRP

gastrin-releasing peptide

LDH

lactate dehydrogenase

RT-PCR

reverse transcription polymerase chain reaction

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • Xinqiu Li
    • 1
  • Litang Zhang
    • 2
  • Xianzhu Ke
    • 1
  • Yuming Wang
    • 1
    Email author
  1. 1.Department of Thyroid, Mammary Gland, and Blood Vessel Surgery, Renmin HospitalHubei University of MedicineShiyanChina
  2. 2.Central Hospital of Coal Group of ZaozhuangZaozhuangChina

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