Biochemistry (Moscow)

, Volume 77, Issue 11, pp 1312–1314

Bacteriolytic activity of human interleukin-2

  • P. A. Levashov
  • S. A. Sedov
  • N. G. Belogurova
  • S. V. Shipovskov
  • A. V. Levashov
Article

Abstract

In this paper we report the discovery of bacteriolytic activity of an immune system cytokine mediator, interleukin-2. Bacteriolytic activity of interleukin-2 was compared with a well-known bacteriolytic enzyme — chicken egg white lysozyme — by monitoring the lysis of the Gram-negative bacterium Escherichia coli, the Gram-positive coccus Micrococcusluteus, and the Gram-positive spore-forming bacillus Bacillus subtilis. It was found that interleukin-2 has greater specificity to the Gram-negative bacterium E. coli than does lysozyme. In contrast to chicken egg white lysozyme, interleukin-2 does not lyse the Gram-positive coccus M. luteus and the Gram-positive spore-forming bacillus B. subtilis. These results give a new understanding of the biological functions of interleukin-2, a regulatory protein that plays a role in oncological and infectious diseases.

Key words

interleukin-2 bacteriolytic activity Escherichia coli 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Levashov, P. A., Sedov, S. A., Shipovskov, S., Belogurova, N. G., and Levashov, A. V. (2010) Anal. Chem., 82, 2161–2163.PubMedCrossRefGoogle Scholar
  2. 2.
    Mitchell, G. J., Nelson, D. C., and Weitz, J. S. (2010) Phys. Biol., 7, 1–12.CrossRefGoogle Scholar
  3. 3.
    Sedov, S. A., Belogurova, N. G., Shipovskov, S., Levashov, A. V., and Levashov, P. A. (2011) Colloids Surf. B, 88, 131–133.CrossRefGoogle Scholar
  4. 4.
    Levashov, P. A., Popov, D. V., Popova, V. M., Zhilenkov, E. L., Morozova, O. A., Belogurova, N. G., Sedov, S. A., Dyatlov, I. A., Klyachko, N. L., and Levashov, A. V. (2010) Biochemistry (Moscow), 75, 1160–1164.CrossRefGoogle Scholar
  5. 5.
    Sedov, S. A., Belogurova, N. G., Shipovskov, S., Semenova, M. V., Gitinov, M. M., Levashov, A. V., and Levashov, P. A. (2012) Bioorg. Khim., 38, 274–281.Google Scholar
  6. 6.
    Bubnova, N. A., and Egorova, V. N. (2010) Generalized Experience on the Use of Ronkoleukin (Recombinant Interleukin-2) in Therapy of Surgical Diseases [in Russian], Alter Ego, St. Petersburg.Google Scholar
  7. 7.
    Molchanov, O. E. (2010) The Use of Ronkoleukin (Recombinant Interleukin-2) in Oncology [in Russian], Alter Ego, St. Petersburg.Google Scholar
  8. 8.
    Myasnikov, A. N., and Smirnov, M. N. (1995) Preparation of Human Recombinant Interleukin-2, Patent RU 2031125 C1.Google Scholar
  9. 9.
    Goa, J. (1953) Scand. J. Clin. Lab. Invest., 5, 218–222.PubMedCrossRefGoogle Scholar
  10. 10.
    Levashov, P. A., Sutherland, D. S., Besenbacher, F., and Shipovskov, S. (2009) Analyt. Biochem., 395, 111–112.PubMedCrossRefGoogle Scholar
  11. 11.
    Levashov, P. A., Ovchinnikova, E. D., Afanasiev, M. I., Frid, D. A., Azmuko, A. A., Bespalova, Zh. D., Adamova, I. Yu., Afanasieva, O. I., and Pokrovsky, S. N. (2012) Bioorg. Khim., 38, 46–50.Google Scholar
  12. 12.
    Pyatkin, K. D., and Krivoshein, Yu. S. (1980) Microbiology [in Russian], Meditsina, Moscow.Google Scholar
  13. 13.
    Poletaev, A. B. (2008) Immunophysiology and Immunopathology [in Russian], Medical Informational Agency, Moscow.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • P. A. Levashov
    • 1
  • S. A. Sedov
    • 1
  • N. G. Belogurova
    • 1
  • S. V. Shipovskov
    • 1
  • A. V. Levashov
    • 1
  1. 1.Department of ChemistryLomonosov Moscow State UniversityMoscowRussia

Personalised recommendations