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Biochemistry (Moscow)

, Volume 77, Issue 10, pp 1181–1189 | Cite as

Engineering of substrate specificity of D-amino acid oxidase from the yeast Trigonopsis variabilis: Directed mutagenesis of Phe258 residue

  • N. V. Komarova
  • I. V. Golubev
  • S. V. Khoronenkova
  • T. A. Chubar’
  • V. I. TishkovEmail author
Article

Abstract

Natural D-amino acid oxidases (DAAO) are not suitable for selective determination of D-amino acids due to their broad substrate specificity profiles. Analysis of the 3D-structure of the DAAO enzyme from the yeast Trigonopsis variabilis (TvDAAO) revealed the Phe258 residue located at the surface of the protein globule to be in the entrance to the active site. The Phe258 residue was mutated to Ala, Ser, and Tyr residues. The mutant TvDAAOs with amino acid substitutions Phe258Ala, Phe258Ser, and Phe258Tyr were purified to homogeneity and their thermal stability and substrate specificity were studied. These substitutions resulted in either slight stabilization (Phe258Tyr) or destabilization (Phe258Ser) of the enzyme. The change in half-inactivation periods was less than twofold. However, these substitutions caused dramatic changes in substrate specificity. Increasing the side chain size with the Phe258Tyr substitution decreased the kinetic parameters with all the D-amino acids studied. For the two other substitutions, the substrate specificity profiles narrowed. The catalytic efficiency increased only for D-Tyr, D-Phe, and D-Leu, and for all other D-amino acids this parameter dramatically decreased. The improvement of catalytic efficiency with D-Tyr, D-Phe, and D-Leu for TvDAAO Phe258Ala was 3.66-, 11.7-, and 1.5-fold, and for TvDAAO Phe258Ser it was 1.7-, 4.75-, and 6.61-fold, respectively.

Key words

D-amino acid oxidase site-directed mutagenesis substrate specificity thermal stability 

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • N. V. Komarova
    • 1
    • 2
  • I. V. Golubev
    • 1
    • 3
  • S. V. Khoronenkova
    • 1
    • 3
  • T. A. Chubar’
    • 1
    • 2
    • 3
  • V. I. Tishkov
    • 1
    • 2
    • 3
    Email author
  1. 1.Innovations and High Technologies MSU Ltd.MoscowRussia
  2. 2.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  3. 3.Chemistry FacultyLomonosov Moscow State UniversityMoscowRussia

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