Biochemistry (Moscow)

, Volume 77, Issue 9, pp 1072–1081 | Cite as

In vitro phosphorylation of the N-terminal half of hordeivirus movement protein

  • V. V. Makarov
  • A. Y. Iconnikova
  • M. A. Guseinov
  • V. K. Vishnichenko
  • N. O. KalininaEmail author


The N-terminal half of TGB1 movement protein of poa semilatent hordeivirus, which forms a ribonucleoprotein complex involved in movement of the viral genome in the plant, and its two domains, NTD and ID, are phosphorylated in vitro by a fraction enriched in cell walls from Nicotiana benthamiana. Using a set of protein kinase inhibitors with different specificities, it was found that enzymes possessing activities of casein kinase 1, protein kinase A, and protein kinase C are involved in phosphorylation. Commercial preparations of protein kinases A and C are able to phosphorylate in vitro recombinant proteins corresponding to the N-terminal half of the protein and its domains NTD and ID. Phosphorylation of the NTD has no effect on the efficiency and character of its binding to RNA. However, phosphorylation of the ID leads to a decrease in its RNA-binding activity and in the ability for homological protein-protein interactions.

Key words

hordeivirus TGB1 movement protein domain phosphorylation RNA-binding activity 



bamboo mosaic virus


casein kinase




internal domain




movement protein


nitrocellulose membrane


Ni2+-nitrilotriacetate agarose


N-terminal protein half including the NTD and ID


N-terminal domain


protein kinase


poa semilatent virus


potato virus A


potato virus X



TGB (1–3)

triple gene block encoded proteins (1–3)


tobacco mosaic virus


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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • V. V. Makarov
    • 1
    • 2
  • A. Y. Iconnikova
    • 1
    • 2
  • M. A. Guseinov
    • 3
  • V. K. Vishnichenko
    • 3
  • N. O. Kalinina
    • 1
    Email author
  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Biological FacultyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Institute of Agricultural BiotechnologyRussian Academy of Agricultural SciencesMoscowRussia

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