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Biochemistry (Moscow)

, Volume 77, Issue 8, pp 878–888 | Cite as

Catalytic properties and amino acid sequence of endo-1→3-β-D-glucanase from the marine mollusk Tapes literata

  • A. M. ZakharenkoEmail author
  • M. I. Kusaykin
  • S. N. Kovalchuk
  • V. V. Sova
  • A. S. Silchenko
  • A. A. Belik
  • S. D. Anastyuk
  • Bui Minh Ly
  • V. A. Rasskazov
  • T. N. Zvyagintseva
Article

Abstract

A specific 1→3-β-D-glucanase with molecular mass 37 kDa was isolated in homogeneous state from crystalline style of the commercial marine mollusk Tapes literata. It exhibits maximal activity within the pH range from 4.5 to 7.5 at 45dgC. The 1→3-β-D-glucanase catalyzes hydrolysis of β-1→3 bonds in glucans as an endoenzyme with retention of bond configuration, and it has transglycosylating activity. The K m for hydrolysis of laminaran is 0.25 mg/ml. The enzyme is classified as a glucan endo-(1→3)-β-D-glucosidase (EC 3.2.1.39). The cDNA encoding this 1→3-β-D-glucanase from T. literata was sequenced, and the amino acid sequence of the enzyme was determined. The endo-1→3-β-D-glucanase from T. literata was assigned to the 16th structural family (GHF 16) of O-glycoside hydrolases.

Key words

1→3-β-D-glucanase crystalline style laminaran marine mollusk Tapes literata transglycosylation 

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • A. M. Zakharenko
    • 1
    Email author
  • M. I. Kusaykin
    • 1
  • S. N. Kovalchuk
    • 1
  • V. V. Sova
    • 1
  • A. S. Silchenko
    • 1
  • A. A. Belik
    • 2
  • S. D. Anastyuk
    • 1
  • Bui Minh Ly
    • 3
  • V. A. Rasskazov
    • 1
  • T. N. Zvyagintseva
    • 1
  1. 1.Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of SciencesVladivostokRussia
  2. 2.Far Eastern Federal UniversityVladivostokRussia
  3. 3.Nha Trang Institute of Technology Research and ApplicationNha TrangVietnam

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