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Biochemistry (Moscow)

, Volume 77, Issue 8, pp 857–863 | Cite as

Role of EctR as transcriptional regulator of ectoine biosynthesis genes in Methylophaga thalassica

  • I. I. Mustakhimov
  • A. S. Reshetnikov
  • D. N. Fedorov
  • V. N. KhmeleninaEmail author
  • Y. A. Trotsenko
Article

Abstract

In the halophilic aerobic methylotrophic bacterium Methylophaga thalassica, the genes encoding the enzymes for biosynthesis of the osmoprotectant ectoine were shown to be located in operon ectABC-ask. Transcription of the ect-operon was started from the two promoters homologous to the σ70-dependent promoter of Escherichia coli and regulated by protein EctR, whose encoding gene, ectR, is transcribed from three promoters. Genes homologous to ectR of methylotrophs were found in clusters of ectoine biosynthesis genes in some non-methylotrophic halophilic bacteria. EctR proteins of methylotrophic and heterotrophic halophiles belong to the MarR-family of transcriptional regulators but form a separate branch on the phylogenetic tree of the MarR proteins.

Key words

methylotrophic bacterium, osmoprotectant, ectoine genes, transcriptional regulation 

Abbreviations

DABA

diamino butyric acid

IPTG

isopropyl β-D-1-thiogalactopyranoside

LB

Luria-Bertani medium

ORF

open reading frame

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • I. I. Mustakhimov
    • 1
  • A. S. Reshetnikov
    • 1
  • D. N. Fedorov
    • 1
  • V. N. Khmelenina
    • 1
    Email author
  • Y. A. Trotsenko
    • 1
  1. 1.Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow RegionRussia

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