Biochemistry (Moscow)

, Volume 77, Issue 6, pp 671–678 | Cite as

Cerebral ischemia-reperfusion induces GAPDH S-nitrosylation and nuclear translocation

  • Chong Li
  • Jun-Jun Feng
  • Yong-Ping Wu
  • Guang-Yi ZhangEmail author


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme, plays an important role in glycolysis. It was reported that GAPDH undergoes S-nitrosylation, which facilitated its binding to Siah1 and resulted in nuclear translocation and cell apoptosis. The results of this study show that GAPDH S-nitrosylation, Siah1 binding, translocation to nucleus, and concomitant neuron death occur during the early stages of reperfusion in the rat four-vessel occlusion ischemic model. N-Methyl-D-aspartate receptor antagonist MK801, neuronal nitric oxide synthase inhibitor 7-nitroindazole, or monoamine oxidase-B inhibitor (R)-(-)-deprenyl hydrochloride could inhibit GAPDH S-nitrosylation and translocation and exert neuroprotective effects.

Key words

GAPDH S-nitrosylation Siah1 deprenyl hydrochloride cerebral ischemia 





(R)-(-)-deprenyl hydrochloride




glyceraldehyde-3-phosphate dehydrogenase


monoamine oxidase B


middle cerebral artery occlusion


nitroblue tetrazolium




neuronal nitric oxide synthase


nitric oxide






four-vessel occlusion


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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • Chong Li
    • 1
  • Jun-Jun Feng
    • 1
  • Yong-Ping Wu
    • 2
  • Guang-Yi Zhang
    • 1
    Email author
  1. 1.Jiangsu Province Key Laboratory of Brain Disease Bioinformation and Research Center of Biochemistry and Molecular BiologyXuzhou Medical CollegeXuzhouJiangsu, China
  2. 2.Jiangsu Province Key Laboratory of AnesthesiologyXuzhou Medical CollegeXuzhou, JiangsuChina

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