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Biochemistry (Moscow)

, Volume 77, Issue 6, pp 659–665 | Cite as

Nanoantibodies for detection and blocking of bioactivity of human vascular endothelial growth factor a165

  • S. V. Tillib
  • T. I. Ivanova
  • E. Yu. LyssukEmail author
  • S. S. Larin
  • A. V. Kibardin
  • E. V. Korobko
  • P. N. Vikhreva
  • N. V. Gnuchev
  • G. P. Georgiev
  • I. V. Korobko
Article

Abstract

Nanoantibodies (single-domain antibodies, nanobodies) derived from noncanonical single-chain immunoglobulins provide an attractive tool for in vitro and in vivo diagnostics as well as for development of targeted drugs for clinical use. Nanoantibodies against several clinically important targets have been developed and are actively investigated. However, no development of nanoantibodies against vascular endothelial growth factor VEGF-A165 has been reported. We describe here the generation of nanoantibodies derived from single-chain Bactrian camel immunoglobulins directed against VEGF-A165. We demonstrate that these nanoantibodies are suitable for enzyme-linked immunoassay to quantify human VEGF-A165 as well as for blocking its activity. Our results provide a basis for diagnostic kit development for quantification of VEGF-A165, which emerges as a biomarker useful in various pathological conditions. In addition, the nanoantibodies might be used for development of therapeutic molecules targeting VEGF-A165-dependent pathological neoangiogenesis.

Key words

nanoantibodies human vascular endothelial growth factor VEGF-A165 diagnostics ELISA VEGF-A165 inhibitor 

Abbreviations

ABTS

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt

ELISA

enzyme-linked immunoassay

PBS

phosphate buffered saline

PCR

polymerase chain reaction

TMB

3,3′,5,5′-tetramethylbenzidine

VEGF-A165

vascular endothelial growth factor A165

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • S. V. Tillib
    • 1
  • T. I. Ivanova
    • 1
  • E. Yu. Lyssuk
    • 1
    Email author
  • S. S. Larin
    • 1
  • A. V. Kibardin
    • 1
  • E. V. Korobko
    • 1
  • P. N. Vikhreva
    • 1
  • N. V. Gnuchev
    • 1
  • G. P. Georgiev
    • 1
  • I. V. Korobko
    • 1
  1. 1.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia

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