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Biochemistry (Moscow)

, Volume 77, Issue 2, pp 208–216 | Cite as

Role of NO-synthase in regulation of protein metabolism of stretched rat m. soleus muscle during functional unloading

  • Yu. N. Lomonosova
  • G. R. Kalamkarov
  • A. E. Bugrova
  • T. F. Shevchenko
  • N. L. Kartashkina
  • E. A. Lysenko
  • B. S. Shenkman
  • T. L. NemirovskayaEmail author
Article

Abstract

Gravitational unloading causes atrophy of muscle fibers and can lead to destruction of cytoskeletal and contractile proteins. Along with the atrophic changes, unloaded muscle frequently demonstrates significant shifts in the ratio of muscle fibers expressing fast and slow myosin heavy chain isoforms. Stretching of the m. soleus during hindlimb suspension prevents its atrophy. We supposed that neuronal NO-synthase (NOS) (which is attached to membrane dystrophin-sarcoglycan complex) can contribute to maintenance of protein metabolism in the muscle and prevent its atrophy when m. soleus is stretched. To test this hypothesis, we used Wistar rats (56 animals) in experiments with hindlimb suspension during 14 days. The group of hindlimb suspended rats with stretched m. soleus was injected with L-NAME to block NOS activity. We found that m. soleus mass and its protein content in hindlimb-suspended rats with stretched m. soleus were preserved due to prevention of protein degradation. NOS is involved in maintenance of expression of some muscle proteins. Proliferation of satellite cells in stretched m. soleus may be due to nNOS activity, but maintenance of muscle mass upon stretching is regulated not by NOS alone.

Key words

m. soleus unloading m. soleus stretching L-NAME cytoskeletal proteins nNOS Hsp90 E3 ligase p70S6K 

Abbreviations

atrogin-1/MAFbx

atrogin-1/Muscle Atrophy F-box

CSA

cross section area

DETC

diethyldithiocarbamate

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

HS

hindlimb-suspended rats

HSS

hindlimb-suspended rats with stretched m. soleus

HSSN

hindlimb-suspended rats with stretched m. soleus and L-NAME injection

Hsp90β

90β heat shock proteins

L-NAME

N-nitro-L-arginine methyl ester hydrochloride

MF

muscle fiber

MHC

myosin heavy chain(s)

mTOR

mammalian target of rapamycin

MuRF-1

muscle-specific RING finger protein 1

(n)NOS

(neuronal) NO synthase

p70S6K

p70S6 kinase

P-p70S6K

phosphorylated form of p70S6 kinase

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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • Yu. N. Lomonosova
    • 1
    • 2
  • G. R. Kalamkarov
    • 3
  • A. E. Bugrova
    • 3
  • T. F. Shevchenko
    • 3
  • N. L. Kartashkina
    • 2
  • E. A. Lysenko
    • 2
  • B. S. Shenkman
    • 2
  • T. L. Nemirovskaya
    • 1
    • 2
    Email author
  1. 1.Faculty of Basic MedicineLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute for Bio-Medical ProblemsRussian Academy of SciencesMoscowRussia
  3. 3.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia

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