Biochemistry (Moscow)

, Volume 77, Issue 2, pp 165–170 | Cite as

Fast kinetics of nucleotide binding to Clostridium perfringens family II pyrophosphatase containing CBS and DRTGG domains

  • J. Jämsen
  • A. A. BaykovEmail author
  • R. LahtiEmail author


We earlier described CBS-pyrophosphatase of Moorella thermoacetica (mtCBS-PPase) as a novel phosphohydrolase that acquired a pair of nucleotide-binding CBS domains during evolution, thus endowing the protein with the capacity to be allosterically regulated by adenine nucleotides (Jämsen, J., Tuominen, H., Salminen, A., Belogurov, G. A., Magretova, N. N., Baykov, A. A., and Lahti, R. (2007) Biochem. J., 408, 327–333). We herein describe a more evolved type of CBS-pyrophosphatase from Clostridium perfringens (cpCBS-PPase) that additionally contains a DRTGG domain between the two CBS domains in the regulatory part. cpCBS-PPase retained the ability of mtCBS-PPase to be inhibited by micromolar concentrations of AMP and ADP and activated by ATP and was additionally activated by diadenosine polyphosphates (AP n A) with n > 2. Stopped-flow measurements using a fluorescent nucleotide analog, 2′(3′)-O-(N-methylanthranoyl)-AMP, revealed that cpCBS-PPase interconverts through two different conformations with transit times on the millisecond scale upon nucleotide binding. The results suggest that the presence of the DRTGG domain affords greater flexibility to the regulatory part, allowing it to more rapidly undergo conformational changes in response to binding.

Key words

inorganic pyrophosphatase CBS domain DRTGG domain Clostridia perfringens adenine nucleotides diadenosine polyphosphate 



P1,Pn-diadenosine 5′-polyphosphate with n bridging phosphate residues


cystathionine β-synthase


CBS domain-containing PPase from Clostridium perfringens




CBS domain-containing PPase from Moorella thermoacetica


inorganic pyrophosphatase


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© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  1. 1.Department of Biochemistry and Food ChemistryUniversity of TurkuTurkuFinland
  2. 2.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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