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Biochemistry (Moscow)

, Volume 77, Issue 1, pp 87–91 | Cite as

Structure of the O-specific polysaccharide of the marine bacterium Arenibacter palladensis KMM 3961T containing 2-acetamido-2-deoxy-L-galacturonic acid

  • S. V. TomshichEmail author
  • V. V. Isakov
  • N. A. Komandrova
  • L. S. Shevchenko
Article

Abstract

The O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Arenibacter palladensis type strain KMM 3961T and studied by chemical methods and 1H and 13C NMR spectroscopy including 2D COSY, TOCSY, 1H,13C HSQC, and HMBC experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units containing two mannose residues (Man), one 2-acetamido-2-deoxy-D-galactose residue (D-GalNAc), and one 2-acetamido-2-deoxy-L-galacturonic acid residue (L-GalNAcA) and having the following structure: \(\to 2) - \alpha - D - Manp - (1 \to 6) - \alpha - D - Manp - (1 \to 4) - \alpha - L - GalpNAcA - (1 \to 3) - \beta - D - GalpNAc - (1 \to\).

Key words

Arenibacter palladensis marine bacteria O-specific polysaccharide structure NMR spectroscopy 2-acetamido-2-deoxy-L-galacturonic acid 

Abbreviations

COSY

correlation spectroscopy

HMBC

heteronuclear multiple bond correlation spectroscopy

HSQC

heteronuclear single-quantum coherence spectroscopy

MS

mass spectrometry

OPS

O-specific polysaccharide

TOCSY

total correlation spectroscopy

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References

  1. 1.
    Bernardet, J. F., Nakagava, Y., and Holmes, B. (2002) Int. J. Syst. Evol. Microbiol., 52, 1049–1070.PubMedCrossRefGoogle Scholar
  2. 2.
    Ivanova, E. P., Nedashkovskaya, O. I., Chun, J., Lysenko, A. M., Frolova, G. M., Svetashev, V. I., Vysotskii, M. V., Mikhailov, V. V., Huq, A., and Colwell, R. R. (2001) Int. J. Syst. Evol. Microbiol., 51, 1987–1995.PubMedCrossRefGoogle Scholar
  3. 3.
    Nedashkovskaya, O. I., Suzuki, M., Vysotskii, M. V., and Mikhailov, V. V. (2003) Int. J. Syst. Evol. Microbiol., 53, 1287–1290.PubMedCrossRefGoogle Scholar
  4. 4.
    Nedashkovskaya, O. I., Kim, S. B., Han, S. K., Lysenko, A. M., Mikhailov, V. V., and Bae, K. S. (2004) Int. J. Syst. Evol. Microbiol., 54, 1173–1176.PubMedCrossRefGoogle Scholar
  5. 5.
    Nedashkovskaya, O. I., Vancanneyt, M., Cleenwerck, I., Snauwaert, C., Kim, S. B., Lysenko, A. M., Shevchenko, L. S., Lee, K. H., Park, M. S., Frolova, G. M., Mikhailov, V. V., Bae, K. S., and Swings, J. (2006) Int. J. Syst. Evol. Microbiol., 56, 155–160.PubMedCrossRefGoogle Scholar
  6. 6.
    Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Lee, K. H., Bae, K. S., and Mikhailov, V. V. (2007) Int. J. Syst. Evol. Microbiol., 57, 2655–2659.PubMedCrossRefGoogle Scholar
  7. 7.
    Sun, F., Wang, B., Du, Y., Liu, X., Lai, Q., Li, G., Luo, J., and Shao, Z. (2010) Int. J. Syst. Evol. Microbiol., 60, 78–83.PubMedCrossRefGoogle Scholar
  8. 8.
    Silipo, A., Molinaro, A., Nazarenko, E. L., Sturiale, L., Garozzo, D., Gorshkova, R. P., Nedashkovskaya, O. I., Lanzetta, R., and Parrilli, M. (2005) Carbohydr. Res., 340, 2540–2549.PubMedCrossRefGoogle Scholar
  9. 9.
    Westphal, O., and Jann, K. (1965) Methods Carbohydr. Chem., 5, 83–91.Google Scholar
  10. 10.
    Conrad, H. E. (1972) Methods Carbohydr. Chem., 6, 361–364.Google Scholar
  11. 11.
    Perepelov, A. V., Senchenkova, S. N., Shashkov, A. S., Komandrova, N. A., Tomshich, S. V., Shevchenko, L. S., Knirel, Yu. A., and Kochetkov, N. K. (2000) J. Chem. Soc. Perkin Trans., 1, 363–366.CrossRefGoogle Scholar
  12. 12.
    Micheel, F., and Klemer, A. (1956) Chemie der Zucker und Polysaccharide, Akademische Verlagsgesellschaft Geest & Portig K.-G., Leipzig, p. 400.Google Scholar
  13. 13.
    Bock, K., and Pedersen, C. (1983) Adv. Carbohydr. Chem. Biochem., 41, 27–66.CrossRefGoogle Scholar
  14. 14.
    Bock, K., and Pedersen, C. (1974) J. Chem. Soc. Perkin Trans., 2, 293–297.Google Scholar
  15. 15.
    Jansson, P.-E., Kenne, L., and Widmalm, G. (1989) Carbohydr. Res., 188, 169–191.PubMedCrossRefGoogle Scholar
  16. 16.
    Knirel, Yu. A., Kocharova, N. A., Shashkov, A. S., Kochetkov, N. K., Kholodkova, E. V., and Stanislavsky, E. S. (1987) Eur. J. Biochem., 166, 189–197.PubMedCrossRefGoogle Scholar
  17. 17.
    Lipkind, G. M., Shashkov, A. S., Knirel, Yu. A., Vinogradov, E. V., and Kochetkov, N. K. (1988) Carbohydr. Res., 175, 59–75.PubMedCrossRefGoogle Scholar
  18. 18.
    Shashkov, A. S., Lipkind, G. M., Knirel, Yu. A., and Kochetkov, N. K. (1988) Magn. Reson. Chem., 26, 735–747.CrossRefGoogle Scholar
  19. 19.
    Darakas, E., Hultberg, H., Leontein, K., and Lonngren, J. (1982) Carbohydr. Res., 103, 176–180.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • S. V. Tomshich
    • 1
    Email author
  • V. V. Isakov
    • 1
  • N. A. Komandrova
    • 1
  • L. S. Shevchenko
    • 1
  1. 1.Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of SciencesVladivostokRussia

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