Biochemistry (Moscow)

, Volume 76, Issue 12, pp 1347–1359 | Cite as

Classification and characterization of putative cytochrome P450 genes from Panax ginseng C. A. Meyer

  • Balusamy Sri Renuka Devi
  • Yu-Jin Kim
  • Subramaniyum Sathiyamoorthy
  • Altanzul Khorolragchaa
  • Sathiyaraj Gayathri
  • Shohana Parvin
  • Dong-Uk Yang
  • Senthil Kalai Selvi
  • Ok Ran Lee
  • Sungyoung Lee
  • Deok-Chun YangEmail author


In plants heme containing cytochrome P450 (P450) is a superfamily of monooxygenases that catalyze the addition of one oxygen atom from O2 into a substrate, with a substantial reduction of the other atom to water. The function of P450 families is attributed to chemical defense mechanism under terrestrial environmental conditions; several are involved in secondary and hormone metabolism. However, the evolutionary relationships of P450 genes in Panax ginseng remain largely unknown. In the present study, data mining methods were implemented and 116 novel putative P450 genes were identified from Expressed Sequence Tags (ESTs) of a ginseng database. These genes were classified into four clans and 22 families by sequence similarity conducted at amino acid level. The representative putative P450 sequences of P. ginseng and known P450 family from other plants were used to construct a phylogenetic tree. By comparing with other genomes, we found that most of the P450 genes from P. ginseng can be found in other dicot species. Depending on P450 family functions, seven P450 genes were selected, and for that organ specific expression, abiotic, and biotic studies were performed by quantitative reverse transcriptase-polymerase chain reaction. Different genes were found to be expressed differently in different organs. Biotic stress and abiotic stress transcript level was regulated diversely, and upregulation of P450 genes indicated the involvement of certain genes under stress conditions. The upregulation of the P450 genes under methyl jasmonate and fungal stress justifies the involvement of specific genes in secondary metabolite biosynthesis. Our results provide a foundation for further elucidating the actual function and role of P450 involved in various biochemical pathways in P. ginseng.

Key words

abiotic biotic ESTs gene expression in silico Panax ginseng secondary metabolites 



conserved domain architecture retrieval tool


Expressed Sequence Tags


methyl jasmonate


cytochrome P450


quantitative real time reverse transcriptase-polymerase chain reaction


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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • Balusamy Sri Renuka Devi
    • 1
  • Yu-Jin Kim
    • 1
  • Subramaniyum Sathiyamoorthy
    • 1
  • Altanzul Khorolragchaa
    • 1
  • Sathiyaraj Gayathri
    • 1
  • Shohana Parvin
    • 1
  • Dong-Uk Yang
    • 1
  • Senthil Kalai Selvi
    • 2
  • Ok Ran Lee
    • 1
  • Sungyoung Lee
    • 3
  • Deok-Chun Yang
    • 1
    Email author
  1. 1.Korean Ginseng Center and Ginseng Genetic Resource BankKyung Hee UniversityGyeonggi-doSouth Korea
  2. 2.Avinashilingam University for WomenCoimbatoreIndia
  3. 3.Department of Computer EngineeringKyung Hee UniversitySeocheonSouth Korea

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