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Biochemistry (Moscow)

, Volume 76, Issue 12, pp 1337–1341 | Cite as

Interaction of synthetic peptide octarphin with rat myocardium membranes

  • Y. N. Nekrasova
  • Y. A. Zolotarev
  • E. V. NavolotskayaEmail author
Article

Abstract

A selective agonist of non-opioid β-endorphin receptor synthetic peptide octarphin (TPLVTLFK, specific activity 28 Ci/mmol) was prepared. The [3H]octarphin binding to rat myocardium membranes before and after experimental myocardial infarction (EMI) was studied. It was found that [3H]octarphin with high affinity and specificity binds to non-opioid β-endorphin receptor of rat myocardium membranes before EMI: K d1 value of the [3H]octarphin specific binding to membranes was 1.8 ± 0.2 nM. In 3 h after EMI a sharp lowering in affinity of the binding is observed (K d2 = 13.3 ± 0.4 nM), and in 48 h its almost complete restoration (K d4 = 2.2 ± 0.3 nM). The results indicate participation of non-opioid β-endorphin receptor in the regulation of myocardial activity.

Key words

β-endorphin naloxone peptide receptor myocardium 

Abbreviations

ECG

electrocardiogram

EMI

experimental myocardial infarction

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • Y. N. Nekrasova
    • 1
  • Y. A. Zolotarev
    • 2
  • E. V. Navolotskaya
    • 1
    Email author
  1. 1.Branch of Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesPushchino, Moscow RegionRussia
  2. 2.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia

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