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Biochemistry (Moscow)

, 76:1107 | Cite as

Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides

  • A. G. YakovlevEmail author
  • L. G. Vasilieva
  • A. Y. Shkuropatov
  • V. A. Shuvalov
Article
  • 61 Downloads

Abstract

Primary stage of charge separation and transfer of charges was studied in reaction centers (RCs) of point mutants LL131H and LL131H/LM160H/FM197H of the purple bacterium Rhodobacter sphaeroides by differential absorption spectroscopy with temporal resolution of 18 fsec at 90 K. Difference absorption spectra measured at 0–4 psec delays after excitation of dimer P at 870 nm with 30 fsec step were obtained in the spectral range of 935–1060 nm. It was found that a decay of P* due to charge separation is considerably slower in the mutant RCs in comparison with native RCs of Rba. sphaeroides. Coherent oscillations were found in the kinetics of stimulated emission of the P* state at 940 nm. Fourier analysis of the oscillations revealed a set of characteristic bands in the frequency range of 20–500 cm−1. The most intense band has the frequency of −30 cm−1 in RCs of mutant LL131H and in native RCs and the frequency of ∼100 cm−1 in RCs of the triple mutant. It was found that an absorption band of bacteriochlorophyll anion B A which is registered in the difference absorption spectra of native RCs at 1020 nm is absent in the analogous spectra of the mutants. The results are analyzed in terms of the participation of the BA molecule in the primary electron transfer in the presence of a nuclear wave packet moving along the inharmonic surface of P* potential energy.

Key words

photosynthesis charge separation reaction center wave packet electron transfer 

Abbreviations

ΔA

absorbance change (light minus dark)

BChl

bacteriochlorophyll

BA and BB

monomeric BChl in A- and B-branch, respectively

BPheo

bacteriopheophytin

Em

midpoint redox potential

HA and HB

BPheo in A- and B- branch, respectively

P

primary electron donor, dimer BChl

PA and PB

BChl molecules constituting P

QA and QB

primary and secondary quinine, respectively

RC

reaction center

Rba.

Rhodobacter

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • A. G. Yakovlev
    • 1
    Email author
  • L. G. Vasilieva
    • 2
  • A. Y. Shkuropatov
    • 2
  • V. A. Shuvalov
    • 1
    • 2
  1. 1.Department of Photobiophysics, Belozersky Institute of Chemical and Physical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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