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Biochemistry (Moscow)

, 76:1030 | Cite as

Selenoprotein dSelK in Drosophila elevates release of Ca2+ from endoplasmic reticulum by upregulating expression of inositol 1,4,5-tris-phosphate receptor

  • S. B. Ben
  • Q. Y. Wang
  • L. Xia
  • J. Z. Xia
  • J. Cui
  • J. Wang
  • F. Yang
  • H. Bai
  • M. S. Shim
  • B. J. Lee
  • L. G. SunEmail author
  • C. L. ChenEmail author
Article

Abstract

dSelK (G-rich), a homolog of human and mouse SelK, is one of three selenoproteins in Drosophila melanogaster. It is the only trans-membrane selenoprotein in D. melanogaster integrated into both the endoplasmic reticulum (ER) membrane and the Golgi apparatus. The gene expression profile of Drosophila Schneider 2 (S2) cells after the dsRNA interference (dsRNAi) targeting of dSelK was examined with the GeneChip Drosophila Genome 2.0 Array (Affymetrix), a high-density oligonucleotide microarray encompassing nearly the full Drosophila genome. The results showed that the transcriptional expression of eight genes whose proteins are located on (or related to) the ER or the Golgi apparatus was highly induced or repressed by the dsRNAi treatment. The mRNA levels of the inositol 1,4,5-tris-phosphate receptor (IP3 receptor), whose gene product is integrated into the ER membrane and regulates the release of Ca2+ from the ER to the cytosol, were significantly downregulated. In contrast, the expression of inositol 1,4,5-tris-phosphate kinase 1, which is a cytosolic protein with opposing functions to the IP3 receptor, was significantly upregulated. Quantitative real-time PCR verified these results. The concentration of intracellular free Ca2+ of the Drosophila S2 cells was significantly decreased after the knockdown of dSelK, whereas overexpression of dSelK significantly increased the intracellular free Ca2+ concentration. These results indicate that dSelK in D. melanogaster is involved in regulating the release of Ca2+ from the ER to the cytosol and may play important roles in the signal transduction pathways involving Ca2+ mobilization.

Key words

Drosophila melanogaster selenoprotein dSelK dsRNAi inositol 1,4,5-tris-phosphate receptor Ca2+ 

Abbreviations

dsRNAi

dsRNA interference

ER

endoplasmic reticulum

FI

fluorescence intensity

Gpx1, -4

glutathione peroxidases 1 and 4

IP3 kinase 1

inositol 1,4,5-tris-phosphate kinase 1

IP3 receptor

inositol 1,4,5-tris-phosphate receptor

ROS

reactive oxygen species

RyR

ryanodine receptors

S2

Schneider 2 (cells)

Sep15

15-kDa selenoprotein

SelK, SelM, SelN, SelS, and SelT

selenoproteins K, M, N, S, and T

Tr1

thioredoxin reductase 1

Supplementary material

10541_2011_9474_MOESM1_ESM.pdf (135 kb)
Supplementary material, approximately 134 KB.

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • S. B. Ben
    • 1
    • 2
  • Q. Y. Wang
    • 1
  • L. Xia
    • 1
  • J. Z. Xia
    • 1
  • J. Cui
    • 1
  • J. Wang
    • 1
  • F. Yang
    • 1
  • H. Bai
    • 1
  • M. S. Shim
    • 3
  • B. J. Lee
    • 3
  • L. G. Sun
    • 2
    Email author
  • C. L. Chen
    • 4
    Email author
  1. 1.School of Life ScienceLiaoning UniversityShenyangChina
  2. 2.Department of Biochemistry and Molecular Biology, Basic Medical CollegeChina Medical UniversityShenyangChina
  3. 3.Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and GeneticsSeoul National UniversitySeoulKorea
  4. 4.School of Pharmaceutical ScienceLiaoning UniversityShenyangChina

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