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Biochemistry (Moscow)

, 76:1022 | Cite as

Mitochondria-targeted plastoquinone derivative SkQ1 decreases ischemia-reperfusion injury during liver hypothermic storage for transplantation

  • D. V. CherkashinaEmail author
  • I. A. Sosimchik
  • O. A. Semenchenko
  • V. V. Volina
  • A. Yu. PetrenkoEmail author
Article

Abstract

The ability of the mitochondria-targeted plastoquinone derivative 10-(6′-plastoquinonyl)decyl triphenylphosphonium (SkQ1) to decrease ischemia-reperfusion injury in isolated liver during hypothermic storage (HS) was studied. Rat liver was stored for 24 h at 4°C without or in the presence of 1 μM SkQ1 with following reperfusion for 60 min at 37°C. The presence in the storage medium of SkQ1 significantly decreased spontaneous production of reactive oxygen species and intensity of lipid peroxidation in the liver during HS and reperfusion. The GSH level after HS in solution with SkQ1 was reliably higher, but reperfusion leveled this effect. At all stages of experiment the presence of SkQ1 did not prevent the decrease of antioxidant enzyme activities such as catalase, GSH peroxidase, GSH reductase, and glucose-6-phosphate dehydrogenase. The addition of SkQ1 to the storage medium improved energetic function of the liver, as was revealed in increased respiratory control index of mitochondria and ATP level. SkQ1 exhibited positive effect on the liver secretory function and morphology after HS as revealed in enhanced bile flow rate during reperfusion and partial recovery of organ architectonics and state of liver sinusoids and hepatocytes. The data point to promising application of mitochondria-targeted antioxidants for correction of the ischemia-reperfusion injury of isolated liver during long-term cold storage before transplantation.

Key words

mitochondria-targeted antioxidant SkQ1 ischemia-reperfusion injury hypothermic storage of isolated liver free radical processes respiratory activity of mitochondria 

Abbreviations

DHR 123

dihydrorhodamine 123

DNP

2,4-dinitrophenol

G6PDH

glucose-6-phosphate dehydrogenase

HS

hypothermic storage

LPO

lipid peroxidation

MDA

malonic dialdehyde

NR

normothermic reperfusion

RCI

respiratory control index

ROS

reactive oxygen species

SkQ1

10-(6′-plastoquinonyl)decyl triphenylphosphonium

TBA

thiobarbituric acid

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Institute for Problems of Cryobiology and CryomedicineUkrainian National Academy of SciencesKharkovUkraine

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