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Heterogeneous structure of O-antigenic part of lipopolysaccharide of Salmonella telaviv (Serogroup O:28) containing 3-acetamido-3,6-dideoxy-D-glucopyranose

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Abstract

The O-polysaccharide of Salmonella Telaviv was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods (sugar and methylation analyses, Smith degradation, de-O-acetylation) and NMR spectroscopy. The structure of the O-polysaccharide was established. The repeating units that are proximal to the lipopolysaccharide core region mostly have a digalactose side chain and lack glucose, whereas those at the other end of the chain mostly do bear glucose but are devoid of the disaccharide side chain. This is the first structure established for the O-polysaccharide of a Salmonella serogroup O:28 (formerly M) strain characterized by subfactors O281 and O282. Knowledge of this structure and the structure of the O-polysaccharide of Salmonella Dakar (O281, O283) established earlier is crucial for determination of the exact structures associated with subfactors O281, O282, and O283 and elucidation of the genetic basis of the close relationship between Escherichia coli O71 and S. enterica O:28 O-antigens.

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Abbreviations

LPS:

lipopolysaccharide

OPS:

O-polysaccharide

References

  1. Grimont, P. A. D., and Weill, F.-X. (2007) Antigenic Formulae of the Salmonella Serovars, 9th Edn., WHO Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur, Paris.

    Google Scholar 

  2. Luderitz, O., Staub, A. M., and Westphal, O. (1966) Bacteriol. Rev., 30, 192–255.

    PubMed  CAS  Google Scholar 

  3. Lindberg, A. A., and Le Minor, L. (1984) Serology of Salmonella, in Methods in Microbiology, Vol. 15 (Bergan, T., ed.) Academic Press, London, pp. 1–64.

    Google Scholar 

  4. Caroff, M., and Karibian, D. (2003) Carbohydr. Res., 338, 2431–2447.

    Article  PubMed  CAS  Google Scholar 

  5. Lerouge, I., and Vanderleyden, J. (2001) FEMS Microbiol. Rev., 26, 17–47.

    Article  Google Scholar 

  6. Wang, W., Perepelov, A. V., Feng, L., Shevelev, S. D., Wang, Q., Senchenkova, S. N., Han, W., Li, Y., Shashkov, A. S., Knirel, Y. A., Reeves, P. R., and Wang, L. (2007) Microbiology, 153, 2159–2167.

    Article  PubMed  CAS  Google Scholar 

  7. D’Aoust, J.-Y. (1994) Int. J. Food Microbiol., 24, 11–31.

    Article  PubMed  Google Scholar 

  8. Brenner, F. W., Villar, R. G., Angulo, F. J., Tauxe, R., and Swaminathan, B. (2000) J. Clin. Microbiol., 38, 2465–2467.

    PubMed  CAS  Google Scholar 

  9. Gast, R. K. (2007) Avian Dis., 51, 817–828.

    Article  PubMed  Google Scholar 

  10. Wang, L., and Reeves, P. R. (2000) J. Bacteriol., 182, 5256–5261.

    Article  PubMed  CAS  Google Scholar 

  11. Hu, B., Perepelov, A. V., Liu, B., Shevelev, S. D., Guo, D., Senchenkova, S. N., Shashkov, A. S., Feng, L., Knirel, Y. A., and Wang, L. (2010) FEMS Immunol. Med. Microbiol., 59, 161–169.

    PubMed  CAS  Google Scholar 

  12. Clark, C. G., Grant, C. C. R., Trout-Yakel, K. M., Tabor, H., Ng, L.-K., Rahn, K., Franklin, K., and Kropinski, A. M. (2010) Int. J. Microbiol., 2010, 209291.

    PubMed  Google Scholar 

  13. Luderitz, O., Ruschmann, E., Westphal, O., Raff, R., and Wheat, R. (1967) J. Bacteriol., 93, 1681–1687.

    PubMed  CAS  Google Scholar 

  14. Kumirska, J., Szafranek, J., Czerwicka, M., Paszkiewicz, M., Dziadziuszko, H., Kunikowska, D., and Stepnowski, P. (2007) Carbohydr. Res., 342, 2138–2143.

    Article  PubMed  CAS  Google Scholar 

  15. Gerwing, G. J., Kamerling, J. P., and Vliegenthart, J. F. G. (1979) Carbohydr. Res., 77, 1–7.

    Article  Google Scholar 

  16. Leontein, K., Lindberg, B., and Lönngren, J. (1978) Carbohydr. Res., 62, 359–362.

    Article  CAS  Google Scholar 

  17. Hakomori, S.-I. (1964) J. Biochem. (Tokyo), 55, 205–208.

    CAS  Google Scholar 

  18. Waeghe, T. J., Darvill, A. G., McNeil, M., and Albersheim, P. (1983) Carbohydr. Res., 123, 281–304.

    Article  CAS  Google Scholar 

  19. Pritchard, D. G., and Rener, B. P. (1988) Carbohydr. Res., 173, 255–262.

    Article  PubMed  CAS  Google Scholar 

  20. Hounsell, E. F. (1995) Prog. Nucl. Magn. Reson. Spectr., 27, 445–474.

    Article  CAS  Google Scholar 

  21. Duus, J. O., Gotfredsen, C. H., and Bock, K. (2000) Chem. Rev., 100, 4589–4614.

    Article  PubMed  CAS  Google Scholar 

  22. Urbina, F., Nordmark, E.-L., Yang, Z., Weintraub, A., Scheutz, F., and Wildman, G. (2005) Carbohydr. Res., 340, 645–650.

    Article  PubMed  CAS  Google Scholar 

  23. Bock, K., and Pedersen, C. (1983) Adv. Carbohydr. Chem. Biochem., 41, 27–66.

    Article  CAS  Google Scholar 

  24. Perry, M. B., Bundle, D. R., MacLean, L., Perry, J. A., and Griffith, D. W. (1986) Carbohydr. Res., 156, 107–122.

    Article  PubMed  CAS  Google Scholar 

  25. Brandbury, H. J., and Jenkins, G. A. (1984) Carbohydr. Res., 126, 125–156.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Gdansk, Sobieskiego 18, 80-952, Gdansk, Poland

    J. Kumirska, M. Czerwicka, E. A. Lubecka, E. M. Siedlecka & P. Stepnowski

  2. Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdansk, Do Studzienki 38, 80-227, Gdansk, Poland

    H. Dziadziuszko & D. Kunikowska

Authors
  1. J. Kumirska
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  2. H. Dziadziuszko
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  3. M. Czerwicka
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  4. E. A. Lubecka
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  5. D. Kunikowska
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  6. E. M. Siedlecka
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  7. P. Stepnowski
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Corresponding author

Correspondence to J. Kumirska.

Additional information

Published in Russian in Biokhimiya, 2011, Vol. 76, No. 7, pp. 956–968.

This paper is based on a presentation made at the 4th Baltic Conference on Microbial Carbohydrates, Hyytiää Forestry Field Station, Finland, September 19-22, 2010.

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Kumirska, J., Dziadziuszko, H., Czerwicka, M. et al. Heterogeneous structure of O-antigenic part of lipopolysaccharide of Salmonella telaviv (Serogroup O:28) containing 3-acetamido-3,6-dideoxy-D-glucopyranose. Biochemistry Moscow 76, 780–790 (2011). https://doi.org/10.1134/S000629791107008X

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  • DOI: https://doi.org/10.1134/S000629791107008X

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