Biochemistry (Moscow)

, Volume 76, Issue 5, pp 550–554 | Cite as

Promyelocytic leukemia protein interacts with werner syndrome helicase and regulates double-strand break repair in γ-irradiation-induced DNA damage responses

  • Jilai Liu
  • Yi SongEmail author
  • Junjie Qian
  • Bin Liu
  • Yan Dong
  • Baolei Tian
  • Zhixian SunEmail author


We show here that γ-irradiation leads to the translocation of endogenous Werner syndrome helicase (WRN) from nucleoli to nucleoplasmic DNA double strand breaks (DSBs), and WRN plays a role in damage repair. The relocation of WRN after irradiation was perturbed by promyelocytic leukemia protein (PML) knockdown and enhanced by PML IV over-expression. PML IV physically interacted with WRN after irradiation. Amino acids (a.a.) 394 to 433 of PML were necessary for this interaction and the nucleoplasmic translocation of WRN and were involved in DSB repair and cellular sensitivity to γ-irradiation. Taken together, our results provide molecular support for a model in which PML IV physically interacts with and regulates the translocation of WRN for DNA damage repair through its 394–433 a.a. domain.

Key words

PML WRN γ-irradiation DNA damage DNA repair 



ataxia-telangiectasia mutated protein




double-strand break


green fluorescent protein


phosphorylated histone H2AX


human embryo lung fibroblasts


ionizing radiation induced foci


promyelocytic leukemia protein


promyelocytic leukemia nuclear bodies


Werner syndrome helicase


Werner’s syndrome


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Supplementary material

10541_2011_9413_MOESM1_ESM.pdf (361 kb)
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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyBeijing Institute of Radiation MedicineBeijingP. R. China

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