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Biochemistry (Moscow)

, Volume 76, Issue 4, pp 462–466 | Cite as

Correlation between influence of polysaccharides on hydrolase activity and their antiviral effect in tobacco leaves

  • L. A. Lapshina
  • V. P. Nagorskaya
  • A. V. ReunovEmail author
  • A. O. Barabanova
  • N. M. Shevchenko
  • I. M. Yermak
  • T. N. Zvyagintseva
  • L. A. Elyakova
Article

Abstract

The activities of hydrolases (acid phosphatase, RNase, and proteases) in healthy and tobacco mosaic virus-infected leaves of Nicotiana tabacum L. var. Samsun, both untreated and treated with polysaccharides (PS) (1,3;1,6-β-D-glucan, fucoidan, and κ/β-carrageenan), were determined. The PS lead to substantial increase in the hydrolase level. The percentage of viral particles undergoing destructive change also increases in leaves treated with PS 24 h before infection. We suppose that the PS-mediated hydrolase activation promotes intracellular destruction of the viral particles and, thus, comprises one of the PS-induced protective mechanisms limiting intracellular viral accumulation.

Key words

1,3;1,6-β-D-glucan fucoidan κ/β-carrageenan acid phosphatase RNase protease tobacco mosaic virus 

Abbreviations

ER

endoplasmic reticulum

PS

polysaccharide

PTA

phosphotungstic acid

PVX

potato virus X

TMV

tobacco mosaic virus

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • L. A. Lapshina
    • 1
  • V. P. Nagorskaya
    • 1
  • A. V. Reunov
    • 1
    Email author
  • A. O. Barabanova
    • 1
  • N. M. Shevchenko
    • 1
  • I. M. Yermak
    • 1
  • T. N. Zvyagintseva
    • 1
  • L. A. Elyakova
    • 1
  1. 1.Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of SciencesVladivostokRussia

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