Biochemistry (Moscow)

, Volume 76, Issue 4, pp 423–426 | Cite as

Effect of glutathione on growth of the probiotic bacterium Lactobacillus reuteri

  • KiBeom Lee
  • Ho-Jin Kim
  • Beom-Seop Rho
  • Sang-Kee Kang
  • Yun-Jaie ChoiEmail author


Glutathione (GSH) is an abundant nonprotein thiol that plays numerous roles within the cell. Previously, we showed that Lactobacillus salivarius has the capacity to mount a glutathione-mediated acid-tolerance response. In the present work we provide evidence of a requirement for GSH by Lactobacillus reuteri and have studied the role of GSH during cell growth. Medium supplementation with 0.5 mM GSH as the sole sulfur source enhanced cell growth, resulting in an increase in glucose consumption, and increased cell GSH and protein contents compared with levels seen in the absence of supplementation. Moreover, L. reuteri showed enhanced amino acid consumption when grown with 0.5 mM GSH. These findings indicate that glutathione is a nutrient for bacterial growth.

Key words

amino acid consumption bacterial growth glutathione Lactobacillus reuteri protein content 



free amino acid


gastrointestinal tract




de Man-Rogosa-Sharpe


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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • KiBeom Lee
    • 1
  • Ho-Jin Kim
    • 1
  • Beom-Seop Rho
    • 1
  • Sang-Kee Kang
    • 2
  • Yun-Jaie Choi
    • 2
    Email author
  1. 1.Department of BiotechnologySongdo TechnoparkYeonsu-Gu, IncheonRepublic of Korea
  2. 2.Laboratory of Animal Cell Biotechnology, Departmrnt of Agricultural BiotechnologySeoul National UniversitySeoulRepublic of Korea

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