Effect of glutathione on growth of the probiotic bacterium Lactobacillus reuteri
Glutathione (GSH) is an abundant nonprotein thiol that plays numerous roles within the cell. Previously, we showed that Lactobacillus salivarius has the capacity to mount a glutathione-mediated acid-tolerance response. In the present work we provide evidence of a requirement for GSH by Lactobacillus reuteri and have studied the role of GSH during cell growth. Medium supplementation with 0.5 mM GSH as the sole sulfur source enhanced cell growth, resulting in an increase in glucose consumption, and increased cell GSH and protein contents compared with levels seen in the absence of supplementation. Moreover, L. reuteri showed enhanced amino acid consumption when grown with 0.5 mM GSH. These findings indicate that glutathione is a nutrient for bacterial growth.
Key wordsamino acid consumption bacterial growth glutathione Lactobacillus reuteri protein content
free amino acid
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- 2.Reiter, R., and Oram, J. D. (1962) J. Dairy Res., 29, 63–68.Google Scholar
- 3.Chamba, J. F., Duong, C., Fazel, A., and Prost, F. (1994) in Bacteries lactiques-Aspects Fondamentaux, Vol. 1 (De Roisart, H., and Luquet, F. M., eds.) Lavoisier, Paris.Google Scholar
- 4.Konova, N. J., Stepanova, O. N., and Akimova, A. A. (1986) Promyshlennost, 2, 32.Google Scholar
- 7.Douglas, K. T. (1987) in Advances in Enzymology, Vol. 59 (Meister, A., ed.) Wiley, N. Y., pp. 103–167.Google Scholar
- 13.Talaat, I. M., and Aziz, E. E. (2005) Egypt J. Appl. Sci., 20, 218–231.Google Scholar
- 14.Catalino, G. A., Akihisa, K., Toru, S., Hiroshi, S., Suteaki, S., and Ken-Ichi, S. (1992) Appl. Microbiol. Biotechnol., 36, 538–540.Google Scholar