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Biochemistry (Moscow)

, Volume 76, Issue 3, pp 359–365 | Cite as

Major chondroitin sulfate proteoglycans identified in L6J1 myoblast culture

  • I. I. ErmakovaEmail author
  • G. A. Sakuta
  • M. A. Potekhina
  • M. A. Fedorova
  • R. Hoffmann
  • V. I. Morozov
Article

Abstract

The major proteoglycans from L6J1 rat myoblast culture were identified. The proteoglycans were isolated from different constituents of cell culture: culture medium, extracellular matrix (ECM), and myoblasts. To identify their core proteins, the proteoglycans were treated with enzymes specifically digesting chondroitin/dermatan sulfates or chondroitin sulfates. Subsequent electrophoresis and mass spectrometry revealed versican, collagen XII, and inter-α-trypsin inhibitor classified as chondroitin sulfate proteoglycans and biglycan known to be chondroitin/dermatan sulfate proteoglycan. Versican was identified in ECM and the other proteoglycans in the culture medium. Such difference in localization is likely to be a consequence of different biological functions. Versican, collagen XII, and biglycan are synthesized by myoblasts and inter-α-trypsin inhibitor originates from fetal bovine serum (a culture medium component).

Key words

proteoglycans glycosaminoglycans myoblasts 

Abbreviations

ECM

extracellular matrix

FBS

fetal bovine serum

GAGs

glycosaminoglycans

ITI

inter-α-trypsin inhibitor

PGs

proteoglycans

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • I. I. Ermakova
    • 1
    • 2
    Email author
  • G. A. Sakuta
    • 1
  • M. A. Potekhina
    • 1
  • M. A. Fedorova
    • 3
  • R. Hoffmann
    • 3
  • V. I. Morozov
    • 1
    • 4
  1. 1.Department of Cell Cultures, Institute of CytologyRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia
  3. 3.Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Faculty of Chemistry and MineralogyLeipzig UniversityLeipzigGermany
  4. 4.Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia

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